Substrates for adhering, culturing and assaying cells
OwnerRonald A. Faris, Terry T. Goodrich +2inventors
InventorsRonald A. Faris, Terry T. Goodrich, John S. Peanasky, Wanda J. Walczak
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Abstract
The present invention provides substrates useful for culturing cells under low serum or serum free conditions. The substrates are useful for conducting cell-based assays where there is interference from serum proteins. Methods are also provided for using the substrates of the present invention as well as methods for making the substrates of the present invention.
Claims20
A substrate for cell culture and cell-based assays comprising:
- a support;
- a tie layer comprising an an aminoalkylsilane or derivatives thereof attached to the support;
- a synthetic polymer layer attached to the tie layer, the synthetic polymer layer comprising a plurality of ionizable hydrophilic groups, ionizable hydrophobic groups, or combinations;
- wherein at least 50% of the ionizable hydrophilic groups, ionizable hydrophobic groups or combinations are inactivated.
A method for performing cell culture and cell-based assays comprising:
- adhering at least one cell to a substrate in the absence of serum proteins, wherein the substrate comprises a support, a tie layer comprising an aminoalkylsilane or derivatives thereof attached to the support, a synthetic polymer layer attached to the tie layer, the synthetic polymer surface comprising a plurality of ionizable hydrophilic groups, ionizable hydrophobic groups or combinations, wherein at least 50% of the ionizable hydrophilic groups, ionizable hydrophobic groups, or combinations are inactivated;
- culturing the cell on the substrate without serum proteins;
- and
- performing a cell-based assay.
A method of producing a substrate for cell culture and cell-based assays comprising:
- attaching a tie layer to a support, wherein the tie layer comprises an aminoalkylsilsesquioxane or derivatives thereof;
- attaching a synthetic polymer layer to the tie layer, the synthetic polymer surface comprising a plurality of ionizable hydrophilic groups, ionizable hydrophobic groups or a combination;
- and
- irradiating the substrate to inactivate at least 50% of the ionizable hydrophilic groups, ionizable hydrophobic groups or combinations.
Dependencies are inferred from the claim text.