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US 2010/0304422 A1Published application
In-Vitro Model of the Transformation of Metaplasia to Neoplasia
OwnerKiron Dasinventor
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Abstract
This invention relates to a novel in-vitro model of transformation of a benign Barrett's cell line to a neoplastic cell line following repeated exposure to acid and bile over the course of about 65 wks, and to the process of using the model to study the transformation.
Claims7
1) An in-vitro model for the study of the transformation of non-neoplastic cells to neoplastic cells which comprises treating a benign Barrett's cell line with acid and bile repeatedly over a period of from about 34 weeks to about 84 weeks.
6) A process for the study of the transformation of a non-neoplastic cell to a neoplastic cell which comprises
- a) growing a Barrett's cell line in medium for a period of from about 34 weeks to about 84 weeks;
- b) treating the growing cell line with acid and bile repeatedly
- c) harvesting a portion of the cells every 4-6 weeks;
- and
- d) determining the morphological changes, molecular changes and anchorage-independent growth of the cells.
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Dependencies are inferred from the claim text.