Assay
AssigneeBelfast Health and Social Care Trust
InventorsPeter Coyle, Derek Fairley, James Mckenna
Life of this patent
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Abstract
The invention provides a LAMP assay for detection of meningococcal disease, the test comprising at least one nucleic acid primer set capable of detecting Neisseria meningitides in a LAMP based molecular test, the primer set being chosen from the primer sets listed in Table 1 as LAMP SETS 1 to 12 comprising SEQUENCE IDs from ID:1 to ID:69. Each assay consists of a primer set including of one pair of forward (FIP) and reverse (BIP) inner primers, forward (F3) and reverse (B3) outer primers. The assay may also include loop forward (LF) and/or loop back (LB) primers to accelerate the reaction. Neisseria meningitides serotypes A, B, C, Y and W135 can be detected using the assay of the invention.
Claims10
A LAMP assay for detection of meningococcal disease, the test comprising at least one nucleic acid primer set capable of detecting Neisseria meningitidis in a LAMP based molecular test, the primer set being chosen from the primer sets listed in Table 1 as LAMP SETS 1 to 12 comprising SEQUENCE IDs from ID:
- 1 to ID:69.
A method for preparing an assay for the detection of Neisseria meningitidis comprising utilizing a primer as set out in Table 1 as Sequence ID:
- 1 through Sequence ID:69, individually or in combination with any of the other listed primers in the preparation of the assay.
A method for diagnosis of meningococcal disease comprising a LAMP assay, the LAMP assay comprising at least one nucleic acid primer set capable of detecting Neisseria meningitidis in a LAMP based molecular test, the primer set being chosen from the primer sets listed in Table 1 as LAMP SETS 1 to 12 comprising SEQUENCE IDs from ID:
- 1 to ID:69.
A method for the detection of Neisseria meningitidis serotypes A, B, C, Y or W 135 from a sample comprising a LAMP assay for detection of Neisseria meningitidis serotypes A, B, C, Y or W 135, the test comprising at least one nucleic acid primer set capable of detecting Neisseria meningitidis in a LAMP based molecular test, the primer set being chosen from the primer sets listed in Table 1 as LAMP SETS 1 to 12 comprising SEQUENCE IDs from ID:
- 1 to ID:69.
A method of diagnosing meningococcal disease in a patient, the method comprising the steps of taking a sample from the patient, accessing DNA in the sample, or extracting DNA from the sample, using at least one LAMP primer set as set out on Table 1 consisting of primer sequences chosen from sequence IDs 1 to 69 to amplify the DNA and testing for amplification to confirm presence of Neisseria meningitidis.
Dependencies are inferred from the claim text.