Influenza Virus Genome Replication and Transcription System in Yeast Cells
AssigneeUniversity of Tsukuba
InventorsKyosuke Nagata, Tadasuke Naito
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Abstract
A system in which vRNP purified from influenza virus particles is introduced into yeast (Saccharomyces cerevisiae) and the transcription and replication from the viral RNA genome are supported by the yeast. The virus genome replication, the virus gene transcription and the virus protein expression occurring in yeast cells in which a great amount of genetic information is accumulated will permit identifying specific host cell factors that are involved in influenza virus infection, influenza virus growth in host and pathogenicity thus providing a tool and a technique that are essential for the development of anti-influenza virus agents.
Claims16
1-11. (canceled)
A kit for preparing a negative-strand RNA virus genome replication system in yeast, which kit comprises at least yeast cells, a viral RNA genome-viral protein complex (vRNP) purified from negative-strand RNA virus particles, reagents for preparing a yeast cell spheroplast, transfection reagents, and a vector for expressing a negative-strand RNA virus protein.
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