Loading patent…
AssigneeRoche Molecular Systems, Inc.
InventorsYoshihiko Hamamoto, Norio Iizuka, Toshiaki Miura, Toyoki Moribe, Masaaki Oka, Shigeru Tamatsukuri
Estimating the term…
The present invention provides a novel method for detection of liver cancer. This method detects high-sensitively, high-specifically, simply and accurately liver cancer, especially that in early stage by identifying and/or quantifying methylation on particular genes and/or their DNA fragments in clinical specimens, and by combining said methylated DNA values with existing tumor marker values and/or DNA amounts in blood. This invention also detects a precancerous lesion, detects a risk of recurrence after treatment of liver cancer, detects malignancy of liver cancer and monitors progression of liver cancer with time by the same method. As for particular genes, BASP1 gene, SPINT2 gene, APC gene, CCND2 gene, CFTR gene, RASSF1 gene and SRD5A2 gene are mentioned.
A method for detecting the presence and/or amount of methylated cytosine specific to liver cancer on a region containing CpG sequences in BASP1 gene and/or SRD5A2 gene comprising the following steps of (a) to (d):
A method for indicating that a patient is afflicted with cancer of the liver, has an increased risk of cancer of the liver, has a risk of recurrence after a treatment for cancer of the liver, has a malignant cancer of the liver or has a progressing cancer of the liver with time by detecting the presence and/or amount of methylated cytosine specific to liver cancer on regions containing CpG sequences in at least two genes of BASP1 gene, SRD5A2 gene, SPINT2 gene, APC gene, CCND2 gene, CFTR gene or RASSF1 gene in the sample from said patient, said method comprising the following steps of (a) to (d):
16-21. (canceled)
Dependencies are inferred from the claim text.