Methods for Determining Virulence and Invasiveness Among Various Staphylococcus Aureus Strains
OwnerLing-Chuan Chen WUinventor
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Abstract
This invention describes quick diagnostic methods to distinguish CA-MRSA MW2 or CA-MRSA USA300 strains in clinical samples from various sources. Fluorescent labeled primers which amplify 1) two unique agr sequences from each strain, 2) a partial spa gene and 3) a partial pvl gene are packaged into a multiplex PCR kit. A real-time instrumentation to amplify and quantify the PCR amplification products, measured by the change of each of the labeled fluorescent reporter and their thermal melting curve, provides a simple and rapid identification of the most virulent and invasive MRSA strains in the United States.
Claims20
A method for detecting community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) MW2 or USA300 strains from a DNA specimen, which comprises:
- a) Preparing clinical samples;
- b) Obtaining DNA specimens;
- c) Combining said DNA specimens with a plurality of primers, wherein said primers comprises SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15 or SEQ ID NO:16;
- d) Setting up a plurality of conditions for a multiplex PCR amplification reaction to get amplified nucleic acid sequences;
- e) Initiating and repeating the multiplex PCR amplification reaction;
- f) Detecting the presence or the absence of a plurality of amplified nucleic acid sequences;
- and
- g) Quantifying and determining a plurality of amplified nucleic acid sequences.
Dependencies are inferred from the claim text.