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AssigneeVereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patientenzorg
InventorsChristophorus Joannes Lambertus Maria Meijer, Petrus Josephus Ferdinandus Snijders
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The invention describes a consensus PCR based method (i.e. HRE7-PCR) for the simultaneous detection of 14 Human Papilloma Virus types (i.e. HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are classified as (probably) high-risk, relating to the causation of cervical cancer) and a candidate hrHPV type (i.e. HPV 67) using sets of 6 overlapping forward primers and 9 overlapping backward primers that together amplify a fragment of about (215) to (245) base pairs of the E7 open reading frame of these hrHPV types. For the detection of reaction products an EIA format can be used with the aid of a cocktail of type-specific oligoprobes as exemplified herein. Furthermore, we have developed a method for an efficient typing of these (15) HPVs that is compatible with the method for detection. This RLB typing system involves hybridisation of PCR products with immobilised type-specific oligoprobes.
Method for the detection of the presence of one or more hrHPV types, selected from the group consisting of HPV type 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 67 and 68 comprising the steps of:
Method according to claim 14, wherein said plurality of hrHPV probes is substantially complementary to the nucleic acid sequence of the said DNA amplification products from all hrHPVs.
Method for typing of one or more hrHPV types, selected from the group consisting of HPV type 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 67 and 68 comprising the steps of:
Set of primers comprising all the forward and backward primers as listed in FIG. 1.
Detection probe for the detection of one or more hrHPV types, selected from the group consisting of HPV type 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 67 and 68 types, which probes are substantially complementary to a region in the E7 region of at least one but not all of said hrHPV types between nucleotide positions 622 and 847 of HPV 16 and corresponding region of the other hrHPV types.
Type-specific detection probe for the detection of one hrHPV type, selected from the group consisting of HPV type 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 67 and 68, which probe is substantially complementary to a region in the E7 region of exactly one type of a hrHPV type between nucleotide positions 622 and 847 of HPV 16 and corresponding region of the other hrHPV.
Dependencies are inferred from the claim text.