Slowly fermentable soluble dietary fiber
AssigneePurdue Research Foundation
InventorsBruce R. Hamaker, Devin J. Rose, Ali Keshavarzian, Pinthip Rumpagaporn, Osvaldo H. Campanella
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Abstract
The embodiments described herein provide a method for improving bowel health by increasing short chain fatty acid concentration in the colon of a mammal including administering to the mammal a composition having from about 0.1 grams to about 50 grams of a treated bran product. After administration, the treated bran product results in an increased short chain fatty acid production during fermentation in the colon as compared to untreated bran fermented in the colon.
Claims19
A method for improving bowel health by increasing short chain fatty acid concentration in the colon of a mammal comprising:
- administering to a mammal a composition having from about 0.1 grams to about 50 grams of a treated bran product produced from a method comprising:
- (a) contacting a bran with a first alpha-amylase enzyme and a first protease enzyme sufficient to form an enzyme modified bran;
- (b) deactivating said first alpha-amylase and protease enzymes;
- (c) contacting said enzyme modified bran with an alkaline and hydrogen peroxide solution for a time sufficient to produce an alkali-soluble bran;
- (d) isolating said alkali-soluble bran from said alkaline and hydrogen peroxide solution;
- (e) contacting said alkali-soluble bran with a second alpha-amylase enzyme and a second protease enzyme sufficient to form a treated bran;
- (f) deactivating said second alpha-amylase and protease enzymes;
- and
- (g) isolating said treated bran from said second alpha-amylase and pro-tease enzymes to form a treated bran product;
- wherein, after administration, said treated bran product results in an increased short chain fatty acid production during fermentation in the colon as compared to untreated bran fermented in the colon, and the carbohydrate content of said treated bran product is reduced more than untreated bran during fermentation.
A method for improving bowel health by increasing short chain fatty acid concentration in the colon of a mammal comprising:
- administering to a mammal a composition having from about 0.1 grams to about 50 grams of a treated bran product produced from a method comprising:
- (a) contacting a bran with a first alpha-amylase enzyme and a first protease enzyme sufficient to form an enzyme modified bran;
- (b) deactivating said first alpha-amylase and protease enzymes;
- (c) contacting said enzyme modified bran with an alkaline and hydrogen peroxide solution for a time sufficient to produce an alkali-soluble bran;
- (d) separating said alkali-soluble bran from said alkaline and hydrogen peroxide solution;
- and
- (e) isolating said alkali-soluble bran using a first and a second precipitation step to produce a treated bran product, wherein said first ethanol precipitation step uses a first ethanol solution and said second ethanol precipitation step uses a second ethanol solution having an ethanol concentration higher than said first ethanol solution;
- wherein, after administration, said treated bran results in an increased short chain fatty acid production during fermentation in the colon as compared to untreated bran fermented in the colon.
A method for improving bowel health by increasing short chain fatty acid concentration in the colon of a mammal comprising:
- administering to a mammal a composition having from about 0.1 grams to about 50 grams of a bran hydrolyzate product produced from a method comprising:
- (a) contacting a bran with a first alpha-amylase enzyme and a first protease enzyme sufficient to form an enzyme modified bran;
- (b) deactivating said first alpha-amylase and protease enzymes;
- (c) contacting said enzyme modified bran with an alkaline and hydrogen peroxide solution for a time sufficient to produce an alkali-soluble bran;
- (d) separating said alkali-soluble bran from said alkaline and hydrogen peroxide solution;
- (e) isolating said alkali-soluble bran using a first and a second precipitation step to produce an ethanol precipitated alkali-soluble bran, wherein said first ethanol precipitation step uses a first ethanol solution and said second ethanol precipitation step uses a second ethanol solution having an ethanol concentration higher than said first ethanol solution;
- (f) contacting said ethanol precipitated alkali-soluble bran with an endoxylanase sufficient to form a bran hydrolyzate;
- (g) deactivating said endoxylanase;
- and
- (h) isolating said bran hydrolyzate using said first and second precipitation step to produce a bran hydrolyzate product;
- wherein, after administration, said bran hydrolyzate product results in an increased short chain fatty acid production during fermentation in the colon as compared to untreated bran fermented in the colon.
Dependencies are inferred from the claim text.