Novel addl receptor polypeptides, polynucleotides and host cells for recombinant production
AssigneeAcumen Pharmaceuticals, Inc.
InventorJasna Jerecic
Life of this patent
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Abstract
This invention provides a novel receptor expressed on neuronal cells in a developmentally-specific manner. Accordingly, this invention provides the amino acid sequences of selected portions of the receptor and polynucleotides encoding these portions as well as antibodies that bind to the polypeptide portions of the receptor. Compositions and methods for using the compositions are also provided.
Claims96
An isolated receptor polypeptide comprising the amino acid sequence:
- a.
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSH
- GKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLE
- DCRF;
- or
- b. a polypeptide having at least 80% homology to SEQ ID NO. 1.
(canceled)
An isolated receptor polypeptide comprising two non-contiguous binding regions having the amino acid sequences:
- a.
- ASIDGFDQYFRSQTLANNR
- (SEQ ID NO. 3)
- and
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- or
- b. amino acid sequences having at least 65% homology to said two non-contiguous polypeptides.
(canceled)
(canceled)
An isolated receptor complex that comprises a plurality of polypeptides having two or more non-contiguous amino acid sequences of the group:
- a.
- AVIMFANEDDIR;
- (SEQ ID NO. 2)
- b.
- ASIDGFDQYFRSQTLANNR;
- (SEQ ID NO. 3)
- c.
- ASIDGFDQYFR;
- (SEQ ID NO. 4)
- d.
- CTENVPVTDFFVGPVCIIPK;
- (SEQ ID NO. 5)
- e.
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- f.
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- g. a polypeptide having at least 65% homology to an amino acid of SEQ ID NOS. 2 through 7.
(canceled)
13-14. (canceled)
16-17. (canceled)
An isolated polynucleotide encoding two non-contiguous binding regions, the binding regions comprising the amino acid sequences:
- a.
- ASIDGFDQYFRSQTLANNR
- (SEQ ID NO. 3)
- and
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- or
- b. amino acids having at least 65% homology to two or more polypeptides.
(canceled)
An isolated polynucleotide encoding the amino acid sequences:
- a.
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC
- TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- or
- b. an amino acid sequence having at least 60% homology to SEQ ID NO. 8.
(canceled)
An isolated polynucleotide encoding a polypeptide having the amino acid sequences:
- a.
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSH
- GKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLE
- DCRF;
- or
- b. a polypeptide having at least 80% to SEQ ID NO. 1.
(canceled)
An isolated polynucleotide encoding a complex, wherein the complex comprises a plurality of polypeptides having two or more non-contiguous amino acid sequences of the group:
- a.
- AVIMFANEDDIR;
- (SEQ ID NO. 2)
- b.
- ASIDGFDQYFRSQTLANNR;
- (SEQ ID NO. 3)
- c.
- ASIDGFDQYFR;
- (SEQ ID NO. 4)
- d.
- CTENVPVTDFFVGPVCIIPK;
- (SEQ ID NO. 5)
- e.
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- f.
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- g. an amino acid having at least 65% homology to polypeptides a. through f.
25-26. (canceled)
29-30. (canceled)
A composition comprising a polynucleotide encoding the amino acid sequences:
- a.
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLG
- SHGKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIP
- LEDCRF;
- or
- b. an amino acid sequence having at least 70% homology to SEQ ID NO. 1;
- and c. a gene delivery vehicle.
32-33. (canceled)
A composition for administering a polynucleotide encoding a receptor polypeptide, the composition comprising a polynucleotide encoding the amino acid sequences:
- a.
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC
- TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- or
- b. an amino acid sequence having at least 60% homology to SEQ ID NO. 8;
- and
- c. a gene delivery vehicle.
35-36. (canceled)
A composition for administering a polynucleotide, the composition comprising one or more polynucleotides encoding non-contiguous amino acid sequences:
- a.
- ASIDGFDQYFRSQTLANNR
- (SEQ ID NO. 3)
- and
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- or
- b. an amino acid sequence having at least 65% homology to SEQ ID NOS. 3 and 6;
- and
- c. a gene delivery vehicle.
(canceled)
A composition for delivering a polynucleotide encoding a complex, said complex comprising one or more polynucleotides encoding a plurality of polypeptides, the polypeptides having two or more amino acid sequences of the group:
- a.
- AVIMFANEDDIR;
- (SEQ ID NO. 3)
- b.
- ASIDGFDQYFRSQTLANNR;
- (SEQ ID NO. 4)
- c.
- ASIDGFDQYFR;
- (SEQ ID NO. 5)
- d.
- CTENVPVTDFFVGPVCIIPK;
- (SEQ ID NO. 6)
- e.
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 7)
- f.
- HDSHIPLEDCR;
- (SEQ ID NO. 8)
- or
- g. an amino acid sequence having at least 65% homology to the polypeptides a. through f.;
- and
- h. a gene delivery vehicle.
A composition for delivering a polynucleotide encoding a complex, comprising one or more polynucleotides that encode amino acid sequences:
- a.
- ASIDGFDQYFR
- (SEQ ID NO. 4)
- and
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- b. an amino acid having at least 65% homology to SEQ ID NOS. 4 or 7;
- and
- c. a gene delivery vehicle.
41-42. (canceled)
A peptide conjugate comprising a carrier covalently or non-covalently linked to at least one polypeptide of the group:
- a.
- AVIMFANEDDIR;
- (SEQ ID NO. 2)
- b.
- ASIDGFDQYFRSQTLANNR;
- (SEQ ID NO. 3)
- c.
- ASIDGFDQYFR;
- (SEQ ID NO. 4)
- d.
- CTENVPVTDFFVGPVCIIPK;
- (SEQ ID NO. 5)
- e.
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- f.
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- g. a polypeptide having at least 70% homology to the polypeptides a. through f.
(canceled)
(canceled)
A peptide conjugate comprising a polypeptide comprising two non-contiguous amino acids:
- a.
- ASIDGFDQYFRSQTLANNR
- (SEQ ID NO. 3)
- and
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- or
- b. a polypeptide having at least 65% homology to SEQ ID NOS. 3 or 6.
(canceled)
A peptide conjugate comprising an polypeptide comprising amino acids:
- a.
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC
- TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- or
- b. an amino acid sequence having at least 60% homology to SEQ ID NO. 8.
50-51. (canceled)
(canceled)
A peptide conjugate comprising the amino acid sequence:
- a.
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLG
- SHGKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIP
- LEDCRF;
- or
- b. an amino acid sequence having at least 80% homology to SEQ ID NO. 1.
55-56. (canceled)
58-59. (canceled)
A method for preparing a polypeptide, comprising expressing a polynucleotide encoding the amino acid sequences:
- a.
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLG
- SHGKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIP
- LEDCRF;
- or
- b. an amino acid sequence having at least 80% homology to SEQ ID NO. 1, in a prokaryotic or eukaryotic host cell.
61-62. (canceled)
A method for preparing a polypeptide, comprising expressing a polynucleotide that specifically binds two non-contiguous binding regions, the binding regions comprising:
- a.
- ASIDGFDQYFRSQTLANNR
- (SEQ ID NO. 3)
- and
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- or
- b. an amino acid having at least 65% homology to SEQ ID NOS. 3 or 6, in a prokaryotic or eukaryotic host cell.
64-65. (canceled)
A method for preparing a polypeptide, comprising expressing a polynucleotide encoding the amino acid sequences:
- a.
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC
- TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- or
- b. an amino acid sequence having at least 60% homology to SEQ ID NO. 8, in prokaryotic or eukaryotic host cell.
67-68. (canceled)
A method for preparing a complex comprising expressing a polynucleotide that encodes two or more non-contiguous amino acid sequences of the group:
- a.
- AVIMFANEDDIR;
- (SEQ ID NO. 2)
- b.
- ASIDGFDQYFRSQTLANNR;
- (SEQ ID NO. 3)
- c.
- ASIDGFDQYFR;
- (SEQ ID NO. 4)
- d.
- CTENVPVTDFFVGPVCIIPK;
- (SEQ ID NO. 5)
- e.
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- f.
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- g. an amino acid sequence having at least 65% homology to polypeptides a. through f., in a prokaryotic or eukaryotic host cell.
70-71. (canceled)
A method for preparing a complex comprising expressing one or more polynucleotides encoding two non-contiguous amino acid sequences:
- a.
- ASIDGFDQYFR
- (SEQ ID NO. 4)
- and
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- b. an amino acid sequence having at least 70% homology to SEQ ID NOS. 4 or 7, in a prokaryotic or eukaryotic host cell.
73-74. (canceled)
An isolated prokaryotic or eukaryotic host cell comprising a polynucleotide encoding amino acid sequences:
- a.
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSH
- GKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLE
- DCRF;
- or
- b. an amino acid sequence having at least 80% homology to SEQ ID NO. 1.
(canceled)
An isolated prokaryotic or eukaryotic host cell comprising a polynucleotide encoding a polypeptide that binds two non-contiguous binding regions, the binding regions comprising amino acid sequences:
- a.
- ASIDGFDQYFRSQTLANNR
- (SEQ ID NO. 3)
- and
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- or
- b. an amino acid having at least 65% homology to SEQ ID NOS. 3 or 6.
(canceled)
An isolated prokaryotic or eukaryotic host cell comprising a polynucleotide encoding the amino acid sequences:
- a.
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKCT
- ENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- or
- b. an amino acid sequence having at least 60% homology to SEQ ID NO. 8.
(canceled)
An isolated prokaryotic or eukaryotic host cell comprising one or more polynucleotides that encode two or more non-contiguous amino acid sequences of the group:
- a.
- AVIMFANEDDIR;
- (SEQ ID NO. 2)
- b.
- ASIDGFDQYFRSQTLANNR;
- (SEQ ID NO. 3)
- c.
- ASIDGFDQYFR;
- (SEQ ID NO. 4)
- d.
- CTENVPVTDFFVGPVCIIPK;
- (SEQ ID NO. 5)
- e.
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- f.
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- g. an amino acid having at least 70% homology to polypeptides a. through f.
(canceled)
An isolated prokaryotic or eukaryotic host cell comprising polynucleotides encoding two non-contiguous amino acid sequences:
- a.
- ASIDGFDQYFR
- (SEQ ID NO. 4)
- and
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- b. an amino acid having at least 65% homology to SEQ ID NOS. 4 or 7.
84-98. (canceled)
A method of inhibiting expression in a cell of a polypeptide, by contacting said cell with an interfering RNA polynucleotide, wherein said polypeptide is selected from the group
- a. a polypeptide comprising amino acid sequence ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEIA EGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHG KRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR F (SEQ ID NO. 1) or an amino acid sequence having at least 80% homology to SEQ ID NO. 1;
- b. a polypeptide comprising the amino acid sequence AVIMFANEDDIR (SEQ ID NO. 2) or an amino acid sequence having at least 65% homology to SEQ ID NO. 2;
- c. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) or an amino acid sequence having at least 65% homology to SEQ ID NO. 3;
- d. a polypeptide comprising the amino acid sequence ASIDGFDQYFR (SEQ ID NO. 4) or an amino acid sequence having at least 65% homology to SEQ ID NO. 4;
- e. a polypeptide comprising the amino acid sequence CTENVPVTDFFVGPVCIIPK (SEQ ID NO. 5) or an amino acid sequence having at least 65% homology to SEQ ID NO. 5;
- f. a polypeptide comprising the amino acid sequence SSVWDERHDSHIPLEDCR (SEQ ID NO. 6) or an amino acid sequence having at least 65% homology to SEQ ID NO. 6;
- g. a polypeptide comprising the amino acid sequence HDSHIPLEDCR (SEQ ID NO. 7) or an amino acid sequence having at least 65% homology to SEQ ID NO. 7;
- h. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR (SEQ ID NO. 8) or an amino acid sequence having at least 60% homology to SEQ ID NO. 8;
- i. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) and SSVWDERHDSHIPLEDCR (SEQ ID NO. 6) or an amino acid sequence having at least 65% homology to SEQ ID NOS. 3 or 6;
- a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFR (SEQ ID NO. 4) and HDSHIPLEDCR (SEQ ID NO. 7) or an amino acid sequence having at least 65% homology to SEQ ID NOS. 4 or 7;
- k. a polypeptide comprising amino acid sequence
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSH
- GKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLE
- DCRF;
- l. a polypeptide comprising the amino acid sequence AVIMFANEDDIR (SEQ ID NO. 2);
- m. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3);
- n. a polypeptide comprising the amino acid sequence ASIDGFDQYFR (SEQ ID NO. 4);
- o. a polypeptide comprising the amino acid sequence CTENVPVTDFFVGPVCIIPK (SEQ ID NO. 5);
- p. a polypeptide comprising the amino acid sequence SSVWDERHDSHIPLEDCR (SEQ ID NO. 6);
- q. a polypeptide comprising the amino acid sequence HDSHIPLEDCR (SEQ ID NO. 7);
- r. a polypeptide comprising the amino acid sequence
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC
- TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- s. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) and SSVWDERHDSHIPLEDCR (SEQ ID NO. 6);
- and
- t. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFR (SEQ ID NO. 4) and HDSHIPLEDCR (SEQ ID NO. 7).
(canceled)
An antibody that binds a polypeptide comprising amino acid sequences:
- a.
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSH
- GKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLE
- DCRF;
- or
- b. an amino acid sequence having at least 80% homology to SEQ ID NO. 1.
102-106. (canceled)
An antibody that binds two binding regions, the two binding regions being non-contiguous and comprising the amino acid sequences:
- a.
- ASIDGFDQYFRSQTLANNR
- (SEQ ID NO. 3)
- and
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- or
- b. an amino acid sequence at least 65% homologous to SEQ ID NOS. 3 or 6.
108-112. (canceled)
An antibody that binds a polypeptide comprising the amino acid sequences:
- a.
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC
- TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- or
- b. an amino acid sequence having at least 60% homology to SEQ ID NO. 8.
114-118. (canceled)
An antibody that binds a polypeptide comprising two non-contiguous amino acids comprising amino acid sequences:
- a.
- ASIDGFDQYFR
- (SEQ ID NO. 4)
- and
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- b. a polypeptide having at least 65% homology to SEQ ID NOS. 4 or 7.
120-124. (canceled)
An antibody that binds at least one polypeptide of the group:
- a.
- AVIMFANEDDIR;
- (SEQ ID NO. 2)
- b.
- ASIDGFDQYFRSQTLANNR;
- (SEQ ID NO. 3)
- c.
- ASIDGFDQYFR;
- (SEQ ID NO. 4)
- d.
- CTENVPVTDFFVGPVCIIPK;
- (SEQ ID NO. 5)
- e.
- SSVWDERHDSHIPLEDCR;
- (SEQ ID NO. 6)
- f.
- HDSHIPLEDCR;
- (SEQ ID NO. 7)
- or
- g. a polypeptide having at least 65% homology to the polypeptides a. through f.
(canceled)
An isolated antibody that does not bind soluble ADDL and binds a polypeptide selected from the group consisting of:
- a. a polypeptide comprising amino acid sequence ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEIA EGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHG KRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR F (SEQ ID NO. 1) or an amino acid sequence having at least 80% homology to SEQ ID NO. 1;
- b. a polypeptide comprising the amino acid sequence AVIMFANEDDIR (SEQ ID NO. 2) or an amino acid sequence having at least 65% homology to SEQ ID NO. 2;
- c. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) or an amino acid sequence having at least 65% homology to SEQ ID NO. 3;
- e. a polypeptide comprising the amino acid sequence ASIDGFDQYFR (SEQ ID NO. 4) or an amino acid sequence having at least 65% homology to SEQ ID NO. 4;
- f. a polypeptide comprising the amino acid sequence CTENVPVTDFFVGPVCIIPK (SEQ ID NO. 5) or an amino acid sequence having at least 65% homology to SEQ ID NO. 5;
- g. a polypeptide comprising the amino acid sequence SSVWDERHDSHIPLEDCR (SEQ ID NO. 6) or an amino acid sequence having at least 65% homology to SEQ ID NO. 6;
- h. a polypeptide comprising the amino acid sequence HDSHIPLEDCR (SEQ ID NO. 7) or an amino acid sequence having at least 65% homology to SEQ ID NO. 7;
- i. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR (SEQ ID NO. 8) or an amino acid sequence having at least 60% homology to SEQ ID NO. 8;
- j. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) and SSVWDERHDSHIPLEDCR (SEQ ID NO. 6) or an amino acid sequence having at least 65% homology to SEQ ID NOS. 3 or 6;
- k. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFR (SEQ ID NO. 4) and HDSHIPLEDCR (SEQ ID NO. 7) or an amino acid sequence having at least 65% homology to SEQ ID NOS. 4 or 7;
- l. a polypeptide comprising amino acid sequence
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSH
- GKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLE
- DCRF;
- m. a polypeptide comprising the amino acid sequence AVIMFANEDDIR (SEQ ID NO. 2);
- n. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3);
- o. a polypeptide comprising the amino acid sequence ASIDGFDQYFR (SEQ ID NO. 4);
- p. a polypeptide comprising the amino acid sequence CTENVPVTDFFVGPVCIIPK (SEQ ID NO. 5);
- q. a polypeptide comprising the amino acid sequence SSVWDERHDSHIPLEDCR (SEQ ID NO. 6);
- r. a polypeptide comprising the amino acid sequence HDSHIPLEDCR (SEQ ID NO. 7);
- s. a polypeptide comprising the amino acid sequence
- (SEQ ID NO. 8)
- ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC
- TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR;
- t. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) and SSVWDERHDSHIPLEDCR (SEQ ID NO. 6);
- or
- u. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFR (SEQ ID NO. 4) and HDSHIPLEDCR (SEQ ID NO. 7).
136-140. (canceled)
A hybridoma cell line that produces an antibody that does not bind soluble ADDL and binds a polypeptide selected from the group consisting of:
- a. a polypeptide comprising amino acid sequence ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEIA EGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHG KRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR F (SEQ ID NO. 1) or an amino acid sequence having at least 80% homology to SEQ ID NO. 1;
- b. a polypeptide comprising the amino acid sequence AVIMFANEDDIR (SEQ ID NO. 2) or an amino acid sequence having at least 65% homology to SEQ ID NO. 2;
- c. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) or an amino acid sequence having at least 65% homology to SEQ ID NO. 3;
- e. a polypeptide comprising the amino acid sequence ASIDGFDQYFR (SEQ ID NO. 4) or an amino acid sequence having at least 65% homology to SEQ ID NO. 4;
- f. a polypeptide comprising the amino acid sequence CTENVPVTDFFVGPVCIIPK (SEQ ID NO. 5) or an amino acid sequence having at least 65% homology to SEQ ID NO. 5;
- g. a polypeptide comprising the amino acid sequence SSVWDERHDSHIPLEDCR (SEQ ID NO. 6) or an amino acid sequence having at least 65% homology to SEQ ID NO. 6;
- h. a polypeptide comprising the amino acid sequence HDSHIPLEDCR (SEQ ID NO. 7) or an amino acid sequence having at least 65% homology to SEQ ID NO. 7;
- i. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR (SEQ ID NO. 8) or an amino acid sequence having at least 60% homology to SEQ ID NO. 8;
- a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) and SSVWDERHDSHIPLEDCR (SEQ ID NO. 6) or an amino acid sequence having at least 65% homology to SEQ ID NOS. 3 or 6;
- k. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFR (SEQ ID NO. 4) and HDSHIPLEDCR (SEQ ID NO. 7) or an amino acid sequence having at least 65% homology to SEQ ID NOS. 4 or 7;
- l. a polypeptide comprising amino acid sequence
- (SEQ ID NO. 1)
- ARAVIMFANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEI
- AEGAVTILPKRASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSH
- GKRNSHIKKCTENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLE
- DCRF;
- m. a polypeptide comprising the amino acid sequence AVIMFANEDDIR (SEQ ID NO. 2);
- n. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3);
- o. a polypeptide comprising the amino acid sequence ASIDGFDQYFR (SEQ ID NO. 4);
- p. a polypeptide comprising the amino acid sequence CTENVPVTDFFVGPVCIIPK (SEQ ID NO. 5);
- q. a polypeptide comprising the amino acid sequence SSVWDERHDSHIPLEDCR (SEQ ID NO. 6);
- r. a polypeptide comprising the amino acid sequence HDSHIPLEDCR (SEQ ID NO. 7);
- s. a polypeptide comprising the amino acid sequence ASIDGFDQYFRSQTLANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKC TENVPVTDFFVGPVCIIPKTDTKPRSSVWDERHDSHIPLEDCR (SEQ ID NO. 8);
- t. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFRSQTLANNR (SEQ ID NO. 3) and SSVWDERHDSHIPLEDCR (SEQ ID NO. 6);
- and
- u. a polypeptide comprising two non-contiguous binding regions, the binding regions comprising ASIDGFDQYFR (SEQ ID NO. 4) and HDSHIPLEDCR (SEQ ID NO. 7).
145-164. (canceled)
Dependencies are inferred from the claim text.