Conjugation process for pnag and a carrier protein
AssigneeGlaxosmithkline Biologicals SA
InventorsRalph Leon Biemans, Pierre Duvivier, Tomas Maira-Litran
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Abstract
The present application describes a process for conjugating a PNAG which is less than 40% N-acetylated to a carrier protein.
Claims24
A process for conjugating a PNAG which is less than 40% N-acetylated to a carrier protein comprising the steps of:
- a) activating the PNAG by adding a linker comprising a maleimide group to form an activated PNAG;
- b) activating the carrier protein by adding a linker comprising a sulphydryl group to form an activated carrier protein;
- and
- c) reacting the activated PNAG and the activated carrier protein to form a PNAG-carrier protein conjugate.
A process for conjugating a PNAG which is less than 40% N-acetylated to a carrier protein comprising the steps of:
- a) activating the PNAG by adding a linker comprising a sulphydryl group to form an activated PNAG;
- b) activating the carrier protein by adding a linker comprising a maleimide group to form an activated carrier protein;
- and
- c) reacting the activated PNAG and the activated carrier protein to form a PNAG-carrier protein conjugate.
A process for conjugating a PNAG which is less than 40% N-acetylated to a carrier protein comprising the steps of:
- a) activating the PNAG by adding a linker comprising a sulphydryl group to form an activated PNAG;
- b) activating the carrier protein by adding a linker comprising a sulphydryl group to form an activated carrier protein;
- and
- c) reacting the activated PNAG and the activated carrier protein to form a PNAG-carrier protein conjugate.
-9. (canceled)
-19. (canceled)
-28. (canceled)
A PNAG-carrier protein conjugate wherein the PNAG is less than 40% N-acetylated and the PNAG and the carrier protein are joined by a linker comprising a maleimide group bonded to a sulphur atom.
-32. (canceled)
A PNAG-carrier protein conjugate wherein the PNAG is less than 40% N-acetylated and the PNAG and the carrier protein are joined by a linker comprising a sulphur atom bonded to a sulphur atom.
-39. (canceled)
An activated PNAG having less than 40% N-acetylation wherein the PNAG is covalently bonded to a linker comprising a maleimide group.
-45. (canceled)
Dependencies are inferred from the claim text.