Apparatus, method and computer program for fluorescence correlation spectroscopy
AssigneeOlympus Corporation
InventorMitsushiro YAMAGUCHI
Life of this patent
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Abstract
There are provided an apparatus, a method and a computer program for fluorescence correlation spectroscopy (FCS), which can reduce the number of times of fluorescence measurements of control samples as few as possible for a measurement by FCS in detecting existence ratios of the respective components contained in a sample. In the inventive apparatus, method and computer program for detecting an existence ratio of each of components with a fluorescent label contained in a solution sample by FCS, using a value of a ratio of a translational diffusion time of each of the components based upon the knowledge that a ratio of a translational diffusion time of each of the components is conservative under different measurement conditions etc.
Claims21
A fluorescence correlation spectroscopic apparatus capable of detecting an existence ratio of each of components of at least two kinds with a fluorescent label contained in a solution sample, the apparatus comprising:
- a data storage region which memorizes a value of a ratio of a translational diffusion time of each of the components of at least two kinds;
- and
- a detecting portion that detects the existence ratio of each of the components of at least two kinds from an autocorrelation function value of a fluorescence intensity measured with the solution sample, using the memorized value of the ratio of the translational diffusion time of each of the components of at least two kinds.
A method for detecting an existence ratio of each of components of at least two kinds with a fluorescent label contained in a solution sample by fluorescence correlation spectroscopy, comprising:
- (a) detecting the existence ratio of each of the components of at least two kinds from an autocorrelation function value of a fluorescence intensity measured with the solution sample, using a value of a ratio of a translational diffusion time of each of the components of at least two kinds memorized in a data storage region.
A computer program product having a computer readable medium including a program for detecting an existence ratio of each of components of at least two kinds with a fluorescent label contained in a solution sample by fluorescence correlation spectroscopy, wherein a program, when executed by a computer, makes the computer perform:
- (a) detecting the existence ratio of each of the components of at least two kinds from an autocorrelation function value of a fluorescence intensity measured with the solution sample, using a value of a ratio of a translational diffusion time of each of the components of at least two kinds memorized in a data storage region.
Dependencies are inferred from the claim text.