An assay for screening/ranking antioxidants based on their effects on membrane sterol lateral organization
AssigneeTemple University- of the Commonweath System of Higher Education
InventorsParkson Lee-Gau CHONG, Michelle OLSHER, Su-In YOON
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Abstract
A method of screening an antioxidant for potency and/or toxicity in vitro, the method includes contacting the antioxidant with a model system comprising a sterol superlattice formation capable of generating a detectable signal, wherein the detectable signal changes in a parameter representative of an integrity of the sterol superlattice formation; and detecting and/or measuring disruption of the sterol superlattice formation, wherein the disruption is caused by oxidation of sterol upon contacting sterol with a prooxidant and the antioxidant and thereby screening the antioxidant for potency and/or toxicity.
Claims18
A method of screening an antioxidant for potency and/or toxicity in vitro, the method comprising:
- contacting the antioxidant with a model system comprising a sterol superlattice formation capable of generating a detectable signal, wherein the detectable signal changes in a parameter representative of an integrity of the sterol superlattice formation;
- and
- detecting and/or measuring disruption of the sterol superlattice formation, wherein the disruption is caused by oxidation of sterol upon contacting sterol with a prooxidant and the antioxidant and thereby screening the antioxidant for potency and/or toxicity.
A method of screening an antioxidant for potency and/or toxicity, the method comprising detecting and/or measuring disruption of a sterol superlattice formation, wherein the disruption is caused by oxidation of sterol upon contacting sterol with a prooxidant (a free radical generator) and the antioxidant.
A method of identifying an antioxidant activity of a compound, the method comprising detecting and/or measuring disruption of a sterol superlattice formation, wherein the disruption is caused by oxidation of sterol upon contacting sterol with a prooxidant and the compound.
A kit for performing an assay for screening and/or ranking antioxidants for strength and toxicity in vitro, the kit comprising:
- a) a set of calibrating liposomes where each liposome comprises a fluorescent sterol probe and a phospholipid, wherein a mole fraction of the fluorescent sterol probe is varied from 18 mol % to 52 mole %;
- b) an antioxidant;
- and
- c) a prooxidant.
Dependencies are inferred from the claim text.