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US 2010/0249381 A1Published application
Method for Purifying FC-Fusion Proteins
OwnerDavid Delvaille, Thierry Ziegler +1inventors
InventorsDavid Delvaille, Thierry Ziegler, Alex Eon-Duval
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Abstract
The invention relates to a method for the purification of Fc-fusion via blue dye affinity chromatography, in particular for the reduction of the amount of free Fc-moieties in an Fc-fusion proteins preparation.
Claims30
1-33. (canceled)
A method for purifying an Fc-fusion proteins preparation containing a single protein fused to an Fc region from free Fc-moieties present in a fluid comprising said Fc-fusion proteins, the method comprising the steps of:
- (a) loading said fluid on a blue dye affinity chromatography resin;
- (b) washing the resin with a buffer having a pH of about 8.4 to about 8.9 to wash the free Fc-moieties from the resin;
- and
- (c) eluting the Fc-fusion proteins from the resin.
1 more element
A method for purifying a polypeptide comprising:
- (a) subjecting a fluid comprising the polypeptide to Protein A chromatography;
- (b) subjecting the eluate of step (a) to blue dye chromatography;
- (c) subjecting the eluate of step (b) to cation exchange chromatography;
- (d) subjecting the eluate of step (c) to hydrophobic interaction chromatography;
- and
- (e) collecting the flow through of the hydrophobic interaction chromatography of step (d).
3 more elements
A method for purifying a polypeptide comprising:
- (a) subjecting a fluid comprising the polypeptide to Protein A chromatography;
- (b) subjecting the eluate of step (a) to blue dye chromatography;
- (c) subjecting the eluate of step (b) to anion exchange chromatography;
- (d) subjecting the eluate of step (c) to size exclusion chromatography;
- and
- (e) collecting the eluate of the size exclusion chromatography step (d).
3 more elements
Dependencies are inferred from the claim text.