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US 2010/0249377 A1Published application
ENGINEERED HYBIRD PHAGE VECTORS FOR THE DESIGN AND THE GENERATION OF A HUMAN NON-ANTIBODY PEPTIDE OR PROTEIN PHAGE LIBRARY VIA FUSION TO pIX OF M13 PHAGE
AssigneeCentoco Ortho Biotech Inc.
InventorsBryan Wang, Karyn O'Neil
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Abstract
The invention relates to a compositions and methods for generating and using pIX phage display libraries for producing non-antibody peptide or protein proteins or peptides using engineered hybrid phage vectors derived from pIX of M 13 phage
Claims18
An engineered recombinant nucleic acid phage vector for expressing phage display fusion peptides or proteins that bind to selected biologically active ligands, comprising
- a. a recombinant phage leader coding nucleic acid sequence;
- operably linked to:
- b. a recombinant tag, promoter, or selection coding nucleic acid sequence;
- operably linked to:
- c. a recombinant pIX or pVII encoding nucleic acid sequence;
- operably linked to:
- d. a recombinant restriction site;
- operably linked to:
- e. a peptide linker encoding nucleic acid sequence;
- operably linked to a:
- f. a first exogenous peptide or protein encoding sequence that selectively binds to a biologically active ligand;
- g. a pVII encoding nucleic acid sequence;
- h. a native pIX encoding nucleic acid sequence;
- i. a pIII encoding nucleic acid sequence;
- and
- j. a pVI encoding nucleic acid sequence
13 more elements
Dependencies are inferred from the claim text.