NELL Peptide Expression Systems and Bone Formation Activity of NELL Peptide
AssigneeThe Regents of the University of California
InventorsKang Ting, Shunichi Kuroda, Ben Wu
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Abstract
The invention generally relates to a bone growth factor, and more particularly to compositions including NELL1, articles of manufacture including NELL1 and methods of using NELL1 to induce bone formation. This invention also provides methods for the expression and purification of NELL1 and NELL2 peptides.
Claims19
A method of expressing a functional peptide in an insect cell, the method comprising:
- providing a nucleic acid construct including at least a nucleic acid encoding at least a NELL1 peptide in frame with a nucleic acid encoding an insect secretory signal peptide;
- transfecting an insect cell with said nucleic acid construct;
- culturing said insect cell under conditions that permit expression of the NELL1 peptide;
- optionally collecting NELL1 peptide secreted from the cell line;
- optionally substantially purifying the NELL1 peptide;
- and
- optionally testing the activity of the NELL1 peptide.
A nucleic acid construct for expressing a NELL1 peptide in an insect cell, said nucleic acid construct comprising at least a nucleic acid encoding at least a NELL1 peptide in frame with a nucleic acid encoding an insect secretory signal peptide.
A cell line for expressing a functional NELL1 peptide, said cell line including a nucleic acid construct comprising at least a nucleic acid encoding at least a NELL1 peptide in frame with a nucleic acid encoding an insect secretory signal peptide.
A polypeptide comprising a NELL1 peptide and an insect secretory signal peptide.
Dependencies are inferred from the claim text.