Reversed flow through platform for rapid analysis of target analytes with increased sensitivity and specificity and the device thereof
AssigneeDiagCor Bioscience Incorporation Ltd.
InventorJoseph Tam
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Abstract
A reversed flow-through device for rapid analysis of target analytes and method thereof, comprises one or more reaction chambers, each of which comprises one or more membranes for immobilizing capture molecules; controlling elements that can be regulated to maintain the reaction chamber in controlled conditions; connecting elements for connection to a power supply and control unit that can regulate and maintain the controlled conditions; and liquid delivery elements capable of accepting and removing solution, wherein the solution is maintained in a flow direction that flows against gravitational force, thereby providing higher sensitivity of analytes detection.
Claims23
A reversed lateral flow-through apparatus for analytes analysis, comprising:
- (a) one or more reaction chambers, each of which comprises one or more membranes for immobilizing capture molecules capable of capturing target analytes or, one or more arrays of capture probes adapted for capturing target molecules;
- (b) control elements that can be regulated to maintain the reaction chamber in controlled conditions;
- (c) connecting elements for connection to a power supply, a control unit for regulating the control elements to maintain the controlled conditions;
- and
- (d) a liquid delivery arrangement capable of delivering and removing a solution comprising the target analytes to and from the reaction chambers, wherein the liquid delivery arrangement is arranged to cause the solution to flow through the one or more membranes or arrays of capture probes against gravitational force so that target analytes can pass through, react with the capture molecules immobilized on the membrane, or capture probes, and be identified.
2-45. (canceled)
A method of performing a rapid analysis of a target analyte, comprising the steps of:
- (a) obtaining a sample comprising a target analyte;
- (b) applying the sample to an array comprising capture molecules or capture probes that will capture the target analyte on the array, wherein the sample is forced to flow through the array from one end to another end of the array in a flow direction that is against gravitational force;
- and
- (c) detecting the captured target analyte on the array.
Dependencies are inferred from the claim text.