Method and assays for quantitation of acetamide in a composition
AssigneeArete Therapeutics, Inc.
InventorsRichard D. Gless, JR., Rebecca Cosford, Tad Dean, Michael Horton, Dean Marbury, Malcolm Montgomery, Robert M. Scarborough
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Abstract
Disclosed herein are the methods and assays for quantitative determination of acetamide in a composition of a sample.
Claims13
A method for assaying a detectable amount of acetamide in a sample suspected of containing the acetamide, the method comprising:
- (a) preparing a first composition suitable for chromatographic analysis wherein the first composition comprises the sample and a known detectable amount of acetamide-d3;
- (b) extracting any detectable acetamide together with the known detectable amount of acetamide-d3 in the first composition by chromatography to provide for a second composition comprising acetamide-d3 and any detectable acetamide contained in the sample;
- and
- (c) assaying for the presence of the acetamide and the amount thereof, if present, in the second composition by passing a portion of the second composition through a mass spectrometer wherein the presence of the acetamide is confirmed by its molecular ion peak and the amount of the acetamide is determined by a comparison of the intensity of the peak generated by the acetamide to the intensity of the peak generated for the known amount of acetamide-d3.
A method for assaying a detectable amount of acetamide in a sample suspected of containing the acetamide, the method comprising:
- (a) preparing a first composition suitable for chromatographic analysis wherein the first composition comprises the sample;
- (b) extracting any detectable acetamide in the first composition by chromatography to provide for a second composition comprising any detectable acetamide contained in the sample;
- and
- (c) assaying for the presence of the acetamide and the amount thereof, if present, in the second composition by passing a portion of the second composition through a mass spectrometer, wherein the presence of the acetamide is confirmed by its molecular ion peak and the amount of the acetamide is determined by a comparison of the intensity of that peak to a concentration curve.
A method for assaying a detectable amount of acetamide in a sample suspected of containing the acetamide, the method comprising:
- (a) generating a curve which correlates an intensity of a mass spectrum peak to a known concentration of acetamide;
- (b) preparing a first composition suitable for chromatographic analysis wherein the first composition comprises the sample;
- (c) extracting any detectable acetamide in the first composition by chromatography to provide for a second composition comprising any detectable acetamide contained in the sample;
- and
- (d) assaying for the presence of the acetamide and the amount thereof, if present, in the second composition by passing a portion of the second composition through a mass spectrometer, wherein the presence of the acetamide is confirmed by its molecular ion peak and the amount of the acetamide is determined by a comparison of the intensity of that peak to the concentration curve prepared in (a) above.
A composition comprising a sample suspected of containing an acetamide and a known amount of acetamide-d3 wherein the acetamide is at a concentration of less than 500 ppm.
Dependencies are inferred from the claim text.