Method for isolation of inner cell mass and method of preparation of embryonic stem cell lines using inner cell mass isolated by the same
OwnerChang Hyun Kiminventor
Life of this patent
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Abstract
A method for isolation of an inner cell mass and a method for preparation of embryonic stem cell lines using the inner cell mass isolated by the same. A blastocyst being free from a zona pellucida removed therefrom is placed on a feeder cell, and a micro cover glass is put on the blastocyst to apply pressure caused by a weight of the micro cover glass, to the blastocyst for a desired time, so that the inner cell mass may be obtained with considerably improved yield compared to conventional methods, and therefore, an embryonic stem cell line may be efficiently established and proliferated.
Claims20
A method for isolation of an inner cell mass, comprising:
- placing a morula or a blastocyst being free from a zona pellucida on a feeder cell or a feeder-independent medium;
- putting a micro cover glass on the morula or the blastocyst to cover and apply pressure to the morula or the blastocyst for a desired time to destroy a trophoblast layer and isolate the inner cell mass from the morula or the blastocyst;
- and
- removing the micro cover glass from the isolated inner cell mass.
A method for preparation of an embryonic stem cell line, comprising:
- placing a morula or a blastocyst being free from a zona pellucida on a feeder cell or a feeder-independent medium;
- putting a micro cover glass on the blastocyst to cover and apply pressure to the morula or the blastocyst for a time sufficient to destroy a trophoblast layer and isolate an inner cell mass from the morula or the blastocyst;
- and
- proliferating the isolated inner cell mass.
A method for isolation of an inner cell mass, comprising:
- placing a blastocyst being free from a zona pellucida on a feeder cell;
- and
- putting a cover on the blastocyst with a space ranging from 4 micrometer to 40 micrometer between the cover and the feeder cell to destroy a trophoblast layer and isolate the inner cell mass from the blastocyst.
Dependencies are inferred from the claim text.