hTERT GENE EXPRESSION REGULATORY GENE
AssigneeNational University Corporation Tottori University
InventorNorimasa Miura
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Abstract
Disclosed is a novel substance capable of regulating the expression of a telomerase reverse transcriptase gene in a cell of a mammal. A gene capable of regulating the expression of hTERT, comprising a nucleotide sequence depicted in SEQ ID No: 1 or 2. The expression of a telomerase reverse transcriptase gene can be inhibited by inhibiting the expression of the gene. By utilizing this mechanism, the expression of a telomerase reverse transcriptase gene can be regulated.
Claims15
A double stranded RNA comprising:
- a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA;
- and
- a second base sequence with a length of 15 bases or longer and 30 bases or shorter, complementary to the first base sequence;
- wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) a base sequence shown in SEQ ID No. 3;
- (b) a base sequence wherein one or a few bases are deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 3;
- and
- (c) a base sequence of a mammal-derived RNA molecule that hybridizes under stringent conditions to an RNA molecule comprising a base sequence complementary to an RNA molecule comprising the base sequence shown in SEQ ID No. 3.
A single stranded RNA comprising:
- a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA;
- and
- a second base sequence with a length of 15 bases or longer and 30 bases or shorter, disposed in the opposite direction to the first base sequence, and complementary to the first base sequence;
- wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) a base sequence shown in SEQ ID No. 3;
- (b) a base sequence wherein one or a few bases are deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 3;
- and
- (c) a base sequence of a mammal-derived RNA molecule that hybridizes under stringent conditions to an RNA molecule comprising a base sequence complementary to an RNA molecule comprising the base sequence shown in SEQ ID No. 3.
An hTERT expression regulatory gene expression vector, comprising a DNA sequence and a vector, wherein the DNA sequence is complementary to a single stranded RNA that comprises:
- a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA;
- and
- a second base sequence with a length of 15 bases or longer and 30 bases or shorter, disposed in the opposite direction to the first base sequence, and complementary to the first base sequence;
- wherein between the first base sequence and the second base sequence, a hairpin loop sequence of 4 bases or more is included;
- and
- the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) a base sequence shown in SEQ ID No. 3;
- (b) a base sequence wherein one or a few bases are deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 3;
- and
- (c) a base sequence of a mammal-derived RNA molecule that hybridizes under stringent conditions to an RNA molecule comprising a base sequence complementary to an RNA molecule comprising the base sequence shown in SEQ ID No. 3.
An RNA composition comprising a plurality of types of double stranded RNA formed by cleaving, with a micro RNA maturation enzyme, a precursor double stranded RNA that comprises:
- an hTERT expression regulatory RNA, and
- an RNA comprising a base sequence complementary to the hTERT expression regulatory RNA,
- wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) a base sequence shown in SEQ ID No. 3;
- (b) a base sequence wherein one or a few bases are deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 3;
- and
- (c) a base sequence of a mammal-derived RNA molecule that hybridizes under stringent conditions to an RNA molecule comprising a base sequence complementary to an RNA molecule comprising the base sequence shown in SEQ ID No. 3.
Dependencies are inferred from the claim text.