Target nucleic acid measuring apparatus
AssigneeOlympus Corporation
InventorsKaneyasu Ohkawa, Yoshinobu Akimoto, Mari Nakamoto, Takeshi Wada
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Abstract
The present invention relates to calculation of an amount equivalent to an initial template amount in a test sample by fitting a theoretical expression to an amount equivalent to an amplification amount of target nucleic acid for each thermal cycle number, wherein the theoretical expression includes an environmental coefficient as an exponent parameter and a parameter of the amount equivalent to the initial template amount, and includes at least one parameter among an amount equivalent to a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient.
Claims15
A target nucleic acid measuring apparatus comprising a storage unit and a control unit,
- wherein the storage unit includes:
- an amplification amount equivalent storage unit that stores an amount equivalent to an amplification amount of target nucleic acid, corresponding to thermal cycle number;
- and
- a theoretical expression storage unit that stores a theoretical expression including an environmental coefficient as an exponent parameter, and a parameter of an amount equivalent to an initial template amount in the test sample, and including at least one parameter among an amount equivalent to a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient, and
- wherein the control unit includes:
- a theoretical expression fitting unit that fits the theoretical expression to the amount equivalent'to the amplification amount of the target nucleic acid for each thermal cycle number stored in the storage unit;
- and
- an initial template amount equivalent calculating unit that calculates the amount equivalent to the initial template amount from the theoretical expression fitted by the theoretical expression fitting unit.
Dependencies are inferred from the claim text.