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AssigneeDsm IP Assets B.V.
InventorsMarie-Gabrielle Beuzelin-Ollivier, Bastian Chevreux, Manuela Dalluegge, Marina Van Gelder, Markus G. Goese, Corina Hauk, Bertus P. Koekman, Connie Lee, Anne F. Mayer, Anja Meury, Nigel J. Mouncey, Dick Schipper, Masako Shinjoh, Christine Toepfer, Adrianus W.H. Vollebregt
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The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
A process for the fermentative production of Vitamin C, wherein a host cell is cultivated under suitable culture conditions that allow the direct production of Vitamin C from a carbon source obtainable from the D-glucose or D-sorbitol metabolization pathway and wherein the genome of said host cell is genetically engineered with DNA sequences comprising the following polynucleotides
A host cell expressing a polypeptide having L-sorbosone dehydrogenase activity with the amino acid sequence according to SEQ ID NO:
Dependencies are inferred from the claim text.