Increasing methionine yield
AssigneeMetabolic Explorer
InventorRainer FIGGE
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Abstract
Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.
Claims29
A method for the production of methionine, a derivative thereof, or a precursor in a fermentative process comprising the following steps:
- culturing a modified microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur, and
- recovering methionine from the culture medium,
- wherein compared to a non-modified microorganism and/or method, the microorganism and/or the method has been modified to present an enhanced methionine/carbon source yield by at least one of the following modifications and combinations thereof:
- decreasing the deformylation of formyl-THF in the microorganism
- decreasing the consumption of PEP in the microorganism
- limiting growth and biomass production of the modified microorganism by limiting or starving the microorganism for one or several inorganic substrate(s) in the culture medium.
A modified microorganism wherein when compared to a non-modified microorganism, said microorganism presents an enhanced methionine/carbon source yield obtained by at least one of the following modifications and combinations thereof:
- decreasing the deformylation of formyl-THF in the microorganism
- decreasing the consumption of PEP in the microorganism.
Dependencies are inferred from the claim text.