Recombinant Microorganism and a Method for Producing Poly-Gamma-Glutamic Acid
OwnerKazuhisa Sawada, Hiroshi Hagiharainventors
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Abstract
A recombinant microorganism having poly-γ-glutamic acid-producing ability, prepared by introducing a Bacillus subtilis pgsB gene or a gene functionally equivalent thereto, and a Bacillus subtilis pgsC gene or a gene functionally equivalent thereto, among a group of genes relating to synthesis of poly-γ-glutamic acid, into a host microorganism, in which no Bacillus subtilis pgsA gene or a gene functionally equivalent thereto is being introduced into the host microorganism; and a method of producing poly-γ-glutamic acid employing the recombinant microorganism.
Claims17
A recombinant microorganism having the ability to produce poly-γ-glutamic acid,
- wherein, the recombinant microorganism is prepared by introducing a Bacillus subtilis pgsB gene or a gene functionally equivalent thereto, and a Bacillus subtilis pgsC gene or a gene functionally equivalent thereto, among a group of genes relating to synthesis of poly-γ-glutamic acid, into a host microorganism, and
- wherein no Bacillus subtilis pgsA gene or a gene functionally equivalent thereto is introduced into the host microorganism.
A method of improving a productivity in poly-γ-glutamic acid production, which comprises employing a recombinant microorganism, which is obtained by introducing a Bacillus subtilis pgsB gene or a gene functionally equivalent thereto, and a Bacillus subtilis pgsC gene or a gene functionally equivalent thereto, among a group of genes relating to the synthesis of poly-γ-glutamic acid, into a host microorganism.
A method of adjusting the molecular weight of poly-γ-glutamic acid, which comprises employing a recombinant microorganism, which is obtained by introducing a Bacillus subtilis pgsB gene or a gene functionally equivalent thereto, and a Bacillus subtilis pgsC gene or a gene functionally equivalent thereto, among a group of genes relating to the synthesis of poly-γ-glutamic acid, into a host microorganism.
A method of adjusting the optical purity of poly-γ-glutamic acid, wherein the L-isomer ratio of the poly-γ-glutamic acid produced is controlled by employing a recombinant microorganism, which is obtained by introducing a Bacillus subtilis pgsB gene or a gene functionally equivalent thereto, and a Bacillus subtilis pgsC gene or a gene functionally equivalent thereto, among a group of genes relating to synthesis of poly-γ-glutamic acid, into a host microorganism.
Dependencies are inferred from the claim text.