Peptide production method
AssigneeKyowa Hakko Bio Co., Ltd.
InventorsKuniki Kino, Toshinobu Arai, Masahiro Kokubo, Makoto Yagasaki
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Abstract
According to the present invention, a protein having peptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having peptide-synthesizing activity using the transformant and the like, a process for producing a peptide using a protein having peptide-synthesizing activity, and a process for producing a peptide using as an enzyme source a culture or the like of a transformant or a microorganism producing a protein having peptide-synthesizing activity are provided.
Claims19
A protein according to any one of the following [1] to [3]:
- [1] a protein having the amino acid sequence shown by any one selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, 10, and 12;
- [2] a protein consisting of an amino acid sequence that has a deletion, a substitution, or an addition of one or more amino acids in the amino acid sequence shown by any one selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, 10, and 12, and having peptide-synthesizing activity;
- and
- [3] a protein consisting of an amino acid sequence that has 80% or more homology with the amino acid sequence shown by any one selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, 10, and 12, and having peptide-synthesizing activity.
A process for producing a peptide, which comprises culturing a microorganism which has an ability to produce a protein having peptide-synthesizing activity and has an ability to produce at least one kind of substrate selected from the group consisting of an amino acid, an amino acid derivative, and a peptide in a medium, allowing the peptide to form and accumulate in the medium, and then recovering the peptide from the medium.
Dependencies are inferred from the claim text.