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US 2010/0248305 A1Published application
Polyamino acid synthetase and gene encoding the same
AssigneeJnc Corporation
InventorsKazuya Yamanaka, Yoshimitu Hamano
Life of this patent
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Abstract
The present invention provides an enzyme which catalyzes amino acid polymerization in the form of a non-ribosomal peptide synthetase (NRPS) and a gene encoding the same.
Claims36
A polynucleotide comprising any one of the following nucleotide sequences:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2;
- (ii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 2;
- (iii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 2;
- (iv) the nucleotide sequence of SEQ ID NO:
- 1;
- (v) a nucleotide sequence encoding a polyamino acid synthetase, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 1 under stringent hybridization conditions;
- and
- (vi) a nucleotide sequence encoding a polyamino acid synthetase, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 1.
10 more elements
A polynucleotide comprising:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2;
- or
- (ii) the nucleotide sequence of SEQ ID NO:
- 1.
2 more elements
A polynucleotide comprising any one of the following nucleotide sequences:
- (i) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (ii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (iii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (iv) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18;
- (v) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 under stringent hybridization conditions;
- and
- (vi) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18.
10 more elements
A polynucleotide comprising:
- (i) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- or
- (ii) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18.
2 more elements
A polyamino acid synthetase comprising any one of the following amino acid sequences:
- (i) the amino acid sequence of SEQ ID NO:
- 2;
- (ii) an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 2;
- and
- (iii) an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 2.
4 more elements
A condensation reaction catalytic domain comprising any one of the following amino acid sequences:
- (i) the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (ii) an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- and
- (iii) an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ. ID NO:
- 13, 15, 17 or 19.
4 more elements
A recombinant vector comprising any one of the following nucleotide sequences:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2;
- (ii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 2;
- (iii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 2;
- (iv) the nucleotide sequence of SEQ ID NO:
- 1;
- (v) a nucleotide sequence encoding a polyamino acid synthetase, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 1 under stringent hybridization conditions;
- and
- (vi) a nucleotide sequence encoding a polyamino acid synthetase, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 1.
10 more elements
A recombinant vector comprising:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2;
- or
- (ii) the nucleotide sequence of SEQ ID NO:
- 1.
2 more elements
A recombinant vector comprising any one of the following nucleotide sequences:
- (i) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (ii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (iii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (iv) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18;
- (v) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 under stringent hybridization conditions;
- and
- (vi) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18.
10 more elements
A recombinant vector comprising:
- (i) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- or
- (ii) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18.
2 more elements
A method for producing a polyamino acid comprising the step of synthesizing the polyamino acid using a polyamino acid synthetase comprising any one of the following amino acid sequences:
- (i) the amino acid sequence of SEQ ID NO:
- 2;
- (ii) an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 2;
- and
- (iii) an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 2.
4 more elements
A method for producing a polyamino acid comprising the step of synthesizing the polyamino acid using a polyamino acid synthetase comprising any one of the following amino acid sequences:
- (i) the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (ii) an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- and
- (iii) an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19.
4 more elements
A primer consisting of a polynucleotide comprising at least 15 contiguous nucleotides in any one of the following nucleotide sequences or complementary sequences thereof:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (ii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (iii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (iv) the nucleotide sequence of SEQ ID NO:
- 1 or a complementary sequence thereof;
- (v) a nucleotide sequence encoding a polyamino acid synthetase, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 1 under stringent hybridization conditions, or a complementary sequence thereof;
- and
- (vi) a nucleotide sequence encoding a polyamino acid synthetase, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 1, or a complementary sequence thereof.
10 more elements
A primer consisting of a polynucleotide comprising at least 15 contiguous nucleotides in any one of the following nucleotide sequences or complementary sequences thereof:
- (i) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- (ii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- (iii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- (iv) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 or a complementary sequence thereof;
- (v) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 under stringent hybridization conditions, or a complementary sequence thereof;
- and
- (vi) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18, or a complementary sequence thereof.
10 more elements
A primer consisting of a polynucleotide comprising at least 15 contiguous nucleotides in any one of the following nucleotide sequences or complementary sequences thereof:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (ii) the nucleotide sequence of SEQ ID NO:
- 1 or a complementary sequence thereof;
- (iii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- and
- (iv) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 or a complementary sequence thereof.
6 more elements
A polynucleotide probe consisting of a nucleotide sequence of at least 15 nucleotides, which can hybridize, under stringent hybridization conditions, to any one of the following nucleotide sequences:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (ii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (iii) a nucleotide sequence encoding a polyamino acid synthetase consisting of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (iv) the nucleotide sequence of SEQ ID NO:
- 1 or a complementary sequence thereof;
- (v) a nucleotide sequence encoding a polyamino acid synthetase, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 1 under stringent hybridization conditions, or a complementary sequence thereof;
- and
- (vi) a nucleotide sequence encoding a polyamino acid synthetase, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 1, or a complementary sequence thereof.
10 more elements
A polynucleotide probe consisting of a nucleotide sequence of at least 15 nucleotides, which can hybridize, under stringent hybridization conditions, to any one of the following nucleotide sequences:
- (i) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- (ii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- (iii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- (iv) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 or a complementary sequence thereof;
- (v) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which can hybridize to a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 under stringent hybridization conditions, or a complementary sequence thereof;
- and
- (vi) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which has at least 65% identity to the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18, or a complementary sequence thereof.
10 more elements
A polynucleotide probe consisting of a nucleotide sequence of at least 15 nucleotides, which can hybridize, under stringent hybridization conditions, to any one of the following nucleotide sequences:
- (i) a nucleotide sequence encoding a polyamino acid synthetase consisting of the amino acid sequence of SEQ ID NO:
- 2 or a complementary sequence thereof;
- (ii) the nucleotide sequence of SEQ ID NO:
- 1 or a complementary sequence thereof;
- (iii) a nucleotide sequence encoding a condensation reaction catalytic domain or a subdomain thereof, which consists of the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19, or a complementary sequence thereof;
- and
- (iv) the nucleotide sequence of SEQ ID NO:
- 12, 14, 16 or 18 or a complementary sequence thereof.
6 more elements
An antibody which specifically binds to a polyamino acid synthetase comprising any one of the following amino acid sequences:
- (i) the amino acid sequence of SEQ ID NO:
- 2;
- (ii) an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 2;
- and
- (iii) an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 2.
4 more elements
An antibody which specifically binds to:
- a condensation reaction catalytic domain or a subdomain thereof, which comprises any one of the following amino acid sequences:
- (i) the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- (ii) an amino acid sequence having deletion, substitution, insertion and/or addition of at least one amino acid residue in the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- and
- (iii) an amino acid sequence having at least 60% identity to the amino acid sequence of SEQ ID NO:
- 13, 15, 17 or 19;
- or a polyamino acid synthetase comprising the domain.
6 more elements
Dependencies are inferred from the claim text.