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US 2010/0248282 A1Published application
DETECTION OF NO BY USING GUANYLYL CYCLASE AND cGMP PRODUCTION AS A READOUT SYSTEM
AssigneeBoehringer Ingelheim International GmbH
InventorFrank BUETTNER
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Abstract
The present invention relates to a method for determining NO enzymatically and its use for the identification of substances which can modulate a nitric oxide synthase activity.
Claims22
-4. (canceled)
A method for determining whether a substance is modulator of nitric oxide synthase activity, wherein the method comprises:
- (a) providing a first solution comprising a nitric oxide synthase and a suitable substrate under conditions which allow synthesis of nitric oxide;
- (b) providing a second solution comprising a nitric oxide synthase and a suitable substrate under conditions which allow synthesis of nitric oxide;
- (c) adding the substance to be tested to the first or the second solution;
- (d) adding guanylyl cyclase and GTP under conditions which allow synthesis of cGMP to the first and the second solution;
- (e) measuring cGMP in the first and the second solution;
- and
- (f) comparing cGMP levels of the first and the second solution.
4 more elements
-12. (canceled)
-22. (canceled)
A kit for determining whether a substance is a modulator of NOS activity comprising:
- (a) guanylyl cyclase,
- (b) GTP, and
- (c) a system for detecting cGMP quantitatively.
A kit for measuring nitric oxid synthase (NOS) activity comprising:
- (a) guanylyl cyclase,
- (b) GTP, and
- (c) a system for detecting cGMP quantitatively.
A kit for detection of nitric oxid (NO) comprising:
- (a) guanylyl cyclase,
- (b) GTP, and
- (c) a system for detecting cGMP quantitatively.
Dependencies are inferred from the claim text.