Substrate probe, enzyme-activity detection method by a multi-dimensional nuclear magnetic resonance method and enzyme-activity imaging method

A substrate peptide-probe for use in measuring protease activity by a multi-dimensional nuclear magnetic resonance method wherein the probe comprising;

• a protease cleavage site as at least one constitutional unit wherein the protease cleavage site is selectively recognized and cleaved by an active-state protease and,
• a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, is present specifically to the protease cleavage site.

A substrate probe for use in measuring enzyme activity by using a multi-dimensional nuclear magnetic resonance method,

• wherein the substrate probe has an enzyme recognition site, which is selectively recognized by an active-state enzyme, as at least one constitutional unit, and
• wherein a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected appears by the enzyme reaction.