Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
AssigneeCaprotec Bioanalytics GmbH
InventorsHubert Kõster, Daniel Paul Little, Suhaib Mahmood Siddiqi, Matthew Peter Grealish, Subramanian Marappan, Chester Frederick Hassman, III, Ping Yip
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Abstract
Capture compounds and collections thereof and methods using the compounds for the analysis of biomolecules are provided. In particular, collections, compounds and methods are provided for analyzing complex protein mixtures, such as the proteome. The compounds are multifunctional reagents that provide for the separation and isolation of complex protein mixtures. Automated systems for performing the methods also are provided.
Claims41
A method for assessing interactions of a molecule Y with targets and non-targets, comprising:
- (a) selecting a molecule whose interactions are to be assessed, and providing a capture compound that presents the molecule, wherein:
- the capture compound has the formula:
- X is an activatable group that, upon exposure to an activating condition, covalently binds to proteins to effect covalent binding of the capture compound to a protein;
- Y is a molecule whose interactions are to be assessed;
- Q is a sorting function for immobilizing or separating the capture compounds;
- Z is a trifunctional group that contains 50 or fewer atoms that presents X, Y and Q;
- m is 1;
- and
- n is 1;
- (b) contacting the capture compound with a sample containing proteins, wherein contacting is effected under conditions in which X is not activated and for a sufficient time for interaction between the capture compounds and proteins in the sample to reach equilibrium, whereby Y interacts target and non-target proteins in the sample;
- (c) exposing the capture compound to a condition that activates X, whereby X forms a covalent linkage with protein(s) in the sample that are interacting with Y to effect capture thereof;
- and
- (d) identifying the captured proteins, wherein the captured proteins comprise targets and non-targets of Y.
Dependencies are inferred from the claim text.