Glycosaminoglycan-coated particles and uses thereof
AssigneeMassachusetts Institute of Technology
InventorsZhihui BAN, Carlos Bosques, Ram Sasisekharan
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Abstract
The present invention is directed to metallic particles coated with glycosaminoglycans and methods for preparing them. Methods of using such glycosaminoglycan-coated metallic particles in biomedical and other applications are also disclosed. In certain embodiments, methods for assaying glycosaminoglycan-degrading activity in biological fluids, test samples, and/or therapeutic formulations using the glycosaminoglycan-coated particles are provided. Such methods may be used, for example, in diagnostic tests for diseases such as cancer, inflammatory diseases, or autoimmune diseases and to test activity of enzymes being developed as therapeutics.
Claims70
A metallic particle coated with a glycosaminoglycan, wherein the largest dimension of the metallic portion of the particle is smaller than 1 μm.
A method of assaying glycosaminoglycan-degrading activity, the method comprising steps of:
- (a) providing metallic particles coated with a glycosaminoglycan, wherein the glycosaminoglycan is capable of being degraded by the activity being assayed for;
- (b) contacting the particles with a sample under suitable conditions to allow the glycosaminoglycan-degrading activity in the sample to degrade the glycosaminoglycans on the surface of the particles;
- and
- (c) determining the glycosaminoglycan-degrading activity in the sample by evaluating the aggregation of the particles.
A method of preparing glycosaminoglycan-coated gold particles, the method comprising steps of:
- (a) mixing a gold precursor with a glycosaminoglycans in water;
- (b) heating the resulting solution;
- (c) adding a reducing agent to the gold-glycosaminoglycan solution with stirring;
- and
- (d) optionally, isolating the resulting glycosaminoglycan-coated gold particles.
A method of preparing glycosaminoglycan-coated cadmium sulfide particles, the method comprising steps of:
- (a) mixing a cadmium precursor with glycosaminoglycan in water;
- and
- (b) adding a source of sulfide to the cadmium-glycosaminoglycan solution.
A method of preparing glycosaminoglycan-coated lead sulfide particles, the method comprising steps of:
- (a) mixing a lead precursor with glycosaminoglycans in water;
- (b) incubating the resulting mixture at room temperature;
- and
- (c) adding a source of sulfide to the lead-glycosaminoglycan solution.
A kit for assaying glycosaminoglycan-degrading activity, comprising
- (a) metallic particles coated with glycosaminoglycans;
- (b) a control sample containing glycosaminoglycan-degrading activity;
- and
- (c) instructions for use.
A particle coated with a glycosaminoglycan selected from the group consisting of chondroitin, chondroitin sulfate, dermatan, dermatan sulfate, keratan, keratan sulfate, heparin, heparan sulfate, and hyaluronan, wherein the particle comprises gold and wherein the largest dimension of the metallic portion (core) of the particle is less than approximately 1 μm.
A particle coated with a glycosaminoglycan, wherein particle comprises gold and is roughly spherical in shape, and wherein the diameter of the metallic portion of the
A particle coated with a glycosaminoglycan, wherein particle comprises gold and is roughly spherical in shape, and wherein the diameter of the metallic portion of the particle is greater than 20 nm and smaller than 1 μm.
Dependencies are inferred from the claim text.