Immunoassay biochip
AssigneeNational Cheng Kung University
InventorsGwo-Bin Lee, Huan-Yao Lei, Yu-Fang Lee, Kang-Yi Lien
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Abstract
The present invention is about a microfluidic chip for rapid detection of different target proteins and a method for using the same. The microfluidic chip utilizes antibody-conjugated magnetic beads to bind to the target proteins to form a magnetic complex, and then use the signal labeled-antibodies that can recognize said magnetic complex. Purifying said magnetic complex by the micro-magnetic field on biochip, and introducing said purified magnetic complex into the fluorescent detection area on the chip to detect the amount of the target protein in said purified complex immediately.
Claims15
An immunoassay biochip, which comprises:
- a mixing chamber;
- a mixer for mixing a fluid in the mixing chamber;
- a purification chamber;
- a first fluidic channel for connecting the mixing chamber and the purification chamber;
- a first bidirectional fluidic channel control unit for controlling the flow directions of a fluid in the first fluidic channel;
- a magnetic field generating unit for attracting magnetic matters in the purification chamber;
- a fluorescence detection unit, which comprises a fluorescence detection fluidic channel, a fluorescence detection area and a unidirectional fluidic channel control unit, wherein the fluorescence detection fluidic channel is used for connecting the purification chamber and the fluorescence detection area, and the unidirectional fluidic channel control unit is used for controlling the flow direction of a fluid in the fluorescence detection fluidic channel so that the fluid flows from the purification chamber into the fluorescence detection area;
- a waste fluidic channel having one end connected to the purification chamber;
- and
- a waste fluidic channel control unit for directing a waste fluid in the purification chamber to flow off via the waste fluidic channel.
An immunoassay biochip, which comprises:
- a mixing chamber;
- a mixer for mixing a fluid in the mixing chamber;
- a purification chamber;
- a first fluidic channel for connecting the mixing chamber and the purification chamber;
- a first bidirectional fluidic channel control unit for controlling the flow directions of a fluid in the first fluidic channel;
- a magnetic field generating unit for attracting magnetic matters in the purification chamber;
- a storage chamber;
- a second fluidic channel for connecting the mixing chamber and the storage chamber;
- a second bidirectional fluidic channel control unit for controlling the flow directions of a fluid in the second fluidic channel;
- at least two fluorescence detection units, which respectively comprises a fluorescence detection fluidic channel, a fluorescence detection area and a unidirectional fluidic channel control unit, wherein the fluorescence detection fluidic channel is used for connecting the purification chamber and the fluorescence detection area, and the unidirectional fluidic channel control unit is used for controlling the flow direction of a fluid in the fluorescence detection fluidic channel so that the fluid flows from the purification chamber into the fluorescence detection area;
- a waste fluidic channel having one end connected to the purification chamber;
- and
- a waste fluidic channel control unit for directing a waste fluid in the purification chamber to flow off via the waste fluidic channel.
Dependencies are inferred from the claim text.