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US 2010/0248252 A1Published application
Methods for analysis of molecular events
AssigneeGenzyme Corporation
InventorsAnthony P. Shuber, Lisa Kann, Duncan Whitney
Life of this patent
Estimating the term…
Abstract
Methods and compositions are provided for detecting the presence of nucleic acid sequence variants in a subpopulation of nucleic acid molecules in a biological sample. These methods are particularly useful for identifying individuals with mutations indicative of cancer.
Claims4
1-33. (canceled)
A method for detecting the presence of one or more variations at a predetermined region in a heterogeneous nucleic acid sample, the method comprising the steps of:
- a) providing a heterogeneous nucleic acid sample comprising a target nucleic acid suspected of containing one or more variant bases at a predetermined region;
- b) providing a primer substantially complementary to a region adjacent to the predetermined region, one or more nucleotides complementary to the corresponding one or more suspected variant bases, one or more chain terminators complementary to corresponding wild type bases in the predetermined region, wherein the one or more nucleotides complementary to the corresponding one or more suspected variant bases are labeled with distinctively detectable signals;
- c) providing one or more polymerases to generate extended primers;
- and
- d) detecting the presence of the distinctively detectable signals in the extended primers, thereby determining the presence of the one or more variant bases in the predetermined region.
2 more elements
Dependencies are inferred from the claim text.