Invitro human embryonic model and a method thereof
AssigneeStempeutics Research Private Limited
InventorsKaushik Dilip Deb, Satish Mahadeo Totey
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Abstract
The present invention relates to the field of stem cells particularly development of a novel human embryonic model using human embryoid bodies obtained from the human embryonic stem cell. The novel human embryonic model disclosed thus can provide a screening assay for determining the toxic activity of the compound and/or drug.
Claims29
1) An in vitro embryonic model comprising spherical smooth-embryoid body (SSE) for determining effect of molecule based on expression of gene HMGB1.
9) An in vitro method for determining effect of molecule on spherical smooth-embryoid body (SSE) comprising acts of:
- a) exposing the molecule to the SSE, and
- b) screening the SSE for expression of gene HMGB1, the effect of the exposure on germ lineages, implantating embryo and on differentiation into tissue;
17) An in vitro embryo implantation model comprising:
- a) coat of extracellular matrix onto support matrix having well(s) or cavity;
- b) layer of endometrial cells onto the extracellular matrix;
- and
- c) spherical smooth-embryoid body (SSE) placed into the well or cavity to determine effect of molecule based on expression of gene HMGB1.
27) An in vitro method of determining effect of lipopolysaccharide (LPS) on embryoid bodies (EBs), said method comprising acts of:
- a. exposing the EBs to the LPS to trigger expression of gene HMGB1 in cytoplasm of the EBs, and
- b. observing silencing of mesoderm induction and functional differentiation in the EBs.
Dependencies are inferred from the claim text.