Detection conjugate
OwnerAlf Mansson, Sven Tagerudinventors
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Abstract
The invention relates to a detection conjugate composed of a filament fragment, e.g. a cytoskeletal filament such as actin filaments or microtubules, and recognition elements bound to this fragment as well as kits comprising said detection conjugate and methods how to use said detection conjugate as well as the use for the detection of one or more compounds present within a sample, such as a biological sample.
Claims18
A detection conjugate comprising
- a. a binding fragment being a cytoskeletal filament, fragments and parts thereof, variants thereof, synthetic versions thereof and chemically modified versions thereof and
- b. at least one recognition element coupled to said fragment covalently or via non-covalent specific affinity coupling.
The detection conjugate according to any of preceding claims, wherein said recognition elements are evenly distributed along said binding fragment.
The detection conjugate according to any of preceding claims, wherein said conjugate comprises at least one reporter molecule.
The detection conjugate according to any of the preceding claims wherein said recognition element and said reporter molecule are evenly distributed along said binding fragments, such as along one side or, alternatively in a helical arrangement along the helical protofilaments of said binding fragments.
The detection conjugate according to any of preceding claims, wherein said binding fragments have a length of from about 100 nm to about 50 m or more.
The detection conjugate according to any of preceding claims wherein different recognition elements are linked/coupled to said binding fragments.
The detection conjugate according to any of the preceding claims, wherein said recognition element is a recognition element for a pathogen, toxin or disease marker.
The detection conjugate according to any of preceding claims, wherein said reporter molecule is selected from the group consisting of coloured substances, fluorochromes, quantum dots, enzymes, gold, silver or magnetic nanoparticles.
One or more detection conjugate(s) according to any of the preceding claims, wherein said conjugates become linked to each other via their bound recognition element(s) in the presence of analyte and thereby cross-link and aggregate.
Dependencies are inferred from the claim text.