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US 2010/0248206 A1Published application
Method of Isolating Stem and Progenitor Cells From Placenta
AssigneeChildren's Hospital & Research Center at Oakland
InventorsFrans A. Kuypers, Vladimir B. Serikov
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Abstract
The present invention provides methods of cryopreserving stem and progenitor cells in a mammalian placenta; and methods of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta. Cells obtained by carrying out the methods can be used in a variety of therapeutic applications.
Claims23
A method for cryopreserving fetal stem and progenitor cells in a mammalian placenta, the method comprising:
- a) perfusing a mammalian placenta with a perfusion solution comprising an anti-coagulant, a vasodilator, and a cryopreservative agent;
- and
- b) freezing the perfused placenta at a temperature below 0° C.
A method of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta, the method comprising:
- a) perfusing a cryopreserved mammalian placenta with a collection solution comprising an agent that induces mobilization of a stem and/or progenitor cell into a blood vessel of the placenta;
- and
- b) collecting the mobilized cells from the blood vessel, wherein the collected cells comprise fetal stem and progenitor cells.
Dependencies are inferred from the claim text.