Nitrite and nitrite-metheme therapy to detoxify stroma-free hemoglobin based blood substitutes
AssigneesThe United States of America, as represented by the Secretary, Department of Health and Human Services, University of Alabama at Birmingham, Wake Forest University
InventorsMark T. Gladwin, Daniel B. Kim-Shapiro, Rakesh P. Patel, Jeffrey Kerby
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Abstract
This disclosure relates to methods of using nitrite to detoxify stroma-free hemoglobin based blood substitutes. In particular, methods are described for using a blood substitute comprised of about equimolar amounts of nitrite and hemoglobin (e.g., nitrite-metHb) to treat, prevent, or ameliorate diseases of the blood in a subject, or as a blood replacement in a subject.
Claims20
A pharmaceutical composition, comprising a ferric (FeIII) heme-containing molecule and nitrite, wherein the molar ratio of nitrite to the heme-containing molecule in the composition is between about 1:2 and about 2:1.
A method of producing a cell-free blood substitute comprising:
- contacting a heme-containing molecule with nitrite,
- wherein the molar ratio of heme-containing molecule and nitrite in the composition is between about 1:2 and about 2:1;
- and
- forming nitrite-bound heme-containing molecules, wherein the nitrite-containing molecules produce dinitrogen trioxide.
Dependencies are inferred from the claim text.