Systems and methods for identifying replikin scaffolds and uses of said replikin scaffolds
OwnerSamuel BOGOCH, Elenore S. Bogoch +2inventors
InventorsSamuel BOGOCH, Elenore S. Bogoch, Samuel Winston Bogoch, Anne Elenore Borsanyi
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Abstract
The present invention provides a new class of peptides related to rapid replication and high human mortality, and their use in diagnosing, preventing and treating disease including vaccines and therapeutics for emerging viral diseases and methods of identifying the new class of peptides and related structures.
Claims31
A method of identifying a Replikin Scaffold in a virus or organism comprising identifying a series of Replikin Scaffold peptides comprising about 16 to about 30 amino acids comprising
- (1) a terminal lysine and a lysine immediately adjacent to said terminal lysine;
- (2) a terminal histidine and a histidine immediately adjacent to said terminal histidine,
- (3) a lysine within about 6 to about 10 amino acids from another lysine;
- and
- (4) at least 6% lysines.
An isolated or synthesized influenza virus peptide comprising from 7 to about 50 amino acids comprising (1) at least one lysine residue located six to ten residues from a second lysine residue wherein at least one lysine residue is a terminal lysine residue;
- (2) at least one histidine residue;
- and (3) at least 6% lysine residues.
An isolated or synthesized influenza virus peptide comprising from 7 to about 50 amino acids comprising (1) at least one lysine residue located six to ten residues from a second lysine residue;
- (2) at least one histidine residue;
- (3) at least 6% lysine residues wherein said isolated or synthesized influenza virus peptide is present in an H5N1 strain of influenza virus.
A preventive or therapeutic virus vaccine comprising at least one isolated or synthesized peptide of influenza virus comprising (1) at least one lysine residue located about six to about ten residues from a second lysine residue;
- (2) at least one histidine residue;
- (3) at least 6% lysine residues.
A method of stimulating an immune system of a subject to produce antibodies to influenza virus comprising administering an effective amount of at least one isolated or synthesized influenza virus Replikin peptide comprising from 7 to about 50 amino acids comprising (1) at least one lysine residue located six to ten residues from a second lysine residue wherein at least one lysine residue is a terminal lysine residue;
- (2) at least one histidine residue;
- and (3) at least 6% lysine residues.
A method of making a preventive or therapeutic virus vaccine comprising identifying a Replikin Scaffold comprising a plurality of Replikin Scaffold peptides comprising about 16 to about 30 amino acids and isolating or synthesizing at least one of said Replikin Scaffold peptides as a preventive or therapeutic virus vaccine wherein said Replikin Scaffold peptides comprise:
- (1) a terminal lysine and a lysine immediately adjacent to said terminal lysine;
- (2) a terminal histidine and a histidine immediately adjacent to said terminal histidine;
- (3) a lysine within about 6 to about 10 amino acids from another lysine;
- and
- (4) at least 6% lysines.
A method comprising:
- applying a plurality of criteria to data representing protein sequences;
- based on the criteria, identifying an arbitrary sub-sequence within the protein sequences;
- and
- outputting the identified sub-sequence to a data file;
- wherein the criteria include:
- a set {a}of amino acids to be included in the sub-sequence;
- a set {b}of amino acids to be excluded from the sub-sequence;
- and
- a minimum and a maximum permissible gap between members of sets {a} and {b}.
A method comprising:
- applying a plurality of criteria to data representing protein sequences;
- based on the criteria, identifying a sub-sequence within the protein sequences, the identified sub-sequence having a predetermined allowed range of distance between lysine amino acids thereof, and a predetermined allowed range of distance between a histidine amino acid and a farthest lysine acid thereof;
- and
- outputting an identified sub-sequence to a data file.
Dependencies are inferred from the claim text.