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US 2010/0247490 A1Published application
SIMIAN E ADENOVIRUSES SAdV-39, -25.2, -26, -30, -37, AND -38
AssigneeThe Trustees of the University of Pennsylvania
InventorsSoumitra Roy, James M. Wilson, Luc H. Vandenberghe
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Abstract
A recombinant vector comprises simian adenovirus SAdV-39, -25.2, -26, -30, -37, and -38 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus SAdV-39, -25.2, -26, -30, -37, and -383 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
Claims22
An adenovirus having a capsid comprising a capsid protein selected from the group consisting of:
- (a) a hexon protein of SAdV-39, amino acids 1 to 940 of SEQ ID NO:
- 11;
- a hexon protein of SAdV-30, amino acids 1 to 938 of SEQ ID NO:
- 108;
- a hexon protein of SAdV-25.2, amino acids 1 to 933 of SEQ ID NO:
- 140;
- a hexon protein of SAdV-37, amino acids 1 to 942 of SEQ ID NO:
- 43;
- a hexon protein of SAdV-38, amino acids 1 to 930 of SEQ ID NO:
- 75;
- a hexon protein of SAdV-26, amino acids 1 to 937 of SEQ ID NO:
- 172;
- (b) a penton protein of SAdV-39, amino acids 1 to 532 of SEQ ID NO:
- 6;
- a penton protein of SAdV-30, amino acids 1 to 533 of SEQ ID NO:
- 103;
- a penton protein of SAdV-25.2, amino acids 1 to 531 of SEQ ID NO:
- 135;
- a penton protein of SAdV-37, amino acids 1 to 542 of SEQ ID NO:
- 38;
- a penton protein of SAdV-38, amino acids 1 to 539 of SEQ ID NO:
- 70;
- a penton protein of SAdV-26, amino acids 1 to 546 of SEQ ID NO:
- 167;
- and
- (c) a fiber protein of SAdV-39, amino acids 1 to 489 of SEQ ID NO:
- 22, a fiber protein of SAdV-30, amino acids 1 to 445 of SEQ ID NO:
- 118;
- a fiber protein of SAdV-25.2, amino acids 1 to 443 of SEQ ID NO:
- 151;
- a fiber protein of SAdV-37, amino acids 1 to 445 of SEQ ID NO:
- 54;
- a fiber protein of SAdV-38, amino acids 1 to 425 of SEQ ID NO:
- 85;
- a fiber protein of SAdV-26, amino acids 1 to 425 of SEQ ID NO:
- 183;
- and
- said capsid encapsidating a heterologous molecule carrying a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.
35 more elements
The adenovirus according to claim 5, wherein said virus has a hybrid capsid.
A recombinant adenovirus having a capsid comprising a hexon containing a fragment of a simian adenovirus hexon protein and a nucleic acid sequence heterologous to the SAdV, wherein the fragment of the SAdV hexon protein is the SAdV hexon protein of SEQ ID NO:11, 108, 140, 43, 75 or 172 with an N-terminal or C-terminal truncation of about 50 amino acids in length or is selected from the group consisting of:
- amino acid residues 125 to 443 of SEQ ID NO:11, 108, 140, 43, 75 or 172;
- amino acid residues 138 to 441 of SEQ ID NO:11, 108, 140, 43, 75 or 172;
- amino acid residues 138 to 163 of SEQ ID NO:11, 108, 140, 43, 75 or 172;
- amino acid residues 170 to 176 of SEQ ID NO:11, 108, 140, 43, 75 or 172;
- and
- amino acid residues 404 to 430 of to SEQ ID NO:
- 11, 108, 140, 43, 75 or 172.
4 more elements
15-16. (canceled)
An isolated simian adenovirus nucleic acid selected from the group consisting of:
- simian adenovirus 39 nucleic acids 1 to 36553 of SEQ ID NO:1 and its complement;
- simian adenovirus 25.2 nucleic acids 1 to 36629 of SEQ ID NO:
- 130 and its complement;
- simian adenovirus 26 nucleic acids 1 to 36628 of SEQ ID NO:
- 162 and its complement;
- simian adenovirus 30 nucleic acids 1 to 36621 of SEQ ID NO:
- 98 and its complement;
- simian adenovirus 37 nucleic acids 1 to 36634 of SEQ ID NO:
- 33 and its complement;
- simian adenovirus 38 nucleic acids 1 to 36494 of SEQ ID NO:
- 65 and its complement.
8 more elements
A vector comprising a simian adenovirus nucleic acid sequence selected from one or more of the group consisting of:
- (a) 5′ inverted terminal repeat (ITR) sequences;
- (b) the adenovirus E1 a region;
- (c) the adenovirus E1 region, or a fragment thereof selected from among the group consisting of the open reading frames for the small T, large T, IX, and IVa2 regions;
- (d) the E2b region, including the open reading frames for the pTP;
- polymerase, and IVa regions;
- (e) the L1 region, or a fragment thereof selected from among the group consisting of the open reading frames for the 52/55 kD protein, and IIIa protein;
- (f) the L2 region, or a fragment thereof selected from the group consisting of the open reading frames for the penton, VII, VI, and X proteins;
- (g) the L3 region, or a fragment thereof selected from the group consisting of the open reading frames for the VI, hexon, or endoprotease;
- (h) the E2a protein, including the open reading frame for the DNA-binding protein (DBP);
- (i) the L4 region, or a fragment thereof selected from the group consisting of the open reading frames for the 100 kD protein, the 33 kD homolog, the 22 kD homolog, and VIII;
- (j) the E3 region, or a fragment thereof selected from the group consisting of the open reading frames for the 12.5 K, CR1-alpha, gp19K, CR1-beta, CR1-gamma, CR1-delta, RID-alpha, RID-beta, and 14.7K;
- (k) the L5 region, or a fragment thereof selected from the open reading frame for the fiber protein;
- (l) the E4 region, or a fragment thereof selected from the group consisting of the open reading frames for the E4 ORF6/7, E4 ORF6, E4 ORF4, E4 ORF3, E4 ORF2, and E4 ORF1;
- and
- (m) the 3′ ITR,
- of simian adenovirus 39, SEQ ID NO:1;
- SAdV-25.2, SEQ ID NO:
- 130;
- simian adenovirus 26, SEQ ID NO:
- 162, simian adenovirus 30, SEQ ID NO:
- 98;
- simian adenovirus 37, SEQ ID NO:
- 33;
- simian adenovirus 38, SEQ ID NO:
- 65.
22 more elements
A composition comprising one or more simian adenovirus protein selected from the group consisting of:
- E1a, selected from the amino acid sequence of SEQ ID NO:30, 127, 159, 62, 95 and 191;
- E1, small T/19K, selected from the amino acid sequence of SEQ ID NO:24, 120, 153, 56, 89, and 185;
- E1, large T/55K, selected from the amino acid sequence of SEQ ID NO:
- 2, 99, 131, 34, 66, and 163;
- IX, selected from the amino acid sequence of SEQ ID NO:3, 100, 132, 35, 67, and 164;
- 52/55D, selected from the amino acid sequence of SEQ ID NO:4, 101, 133, 36, 68, and 165;
- IIIa, selected from the amino acid sequence of SEQ ID NO:5, 102, 134, 37, 69 and 166;
- Penton, selected from the amino acid sequence of SEQ ID NO:6, 103, 135, 38, 70, and 167;
- VII, selected from the amino acid sequence of SEQ ID NO:
- 7, 104, 136, 39, 71, and 168;
- V, selected from the amino acid sequence of SEQ ID NO:
- 8, 105, 137, 40, 72, and 169;
- pX, selected from the amino acid sequence of SEQ ID NO:
- 9, 106, 138, 41, 73, and 170;
- VI, selected from the amino acid sequence of SEQ ID NO:
- 10, 107, 139, 42, 74, and 171;
- Hexon, selected from the amino acid sequence of SEQ ID NO:
- 11, 108, 140, 43, 75, and 172;
- Endoprotease, selected from the amino acid sequence of SEQ ID NO:12, 109, 141, 44, 76, and 173;
- 100 kD, selected from the amino acid sequence of SEQ ID NO:13, 110, 142, 45, 77, and 174;
- 33 kD, selected from the amino acid sequence of SEQ ID NO:
- 32, 129, 161, 64, 97, 97, and 193;
- 22 kD, selected from the amino acid sequence of SEQ ID NO:
- 26, 122, 155, 58, 91, and 187;
- VIII, selected from the amino acid sequence of SEQ ID NO:14, 111, 143, 46, 78, and 175;
- E3/12.5 K, selected from the amino acid sequence of SEQ ID NO:15, 123, 144, 47, 79, and 176;
- CR1-alpha, selected from the amino acid sequence of SEQ ID NO:27, 112, 156, 59, 92, and 188;
- gp19K, selected from the amino acid sequence of SEQ ID NO:16, 124, 145, 48, 87, and 177;
- CR1-beta, selected from the amino acid sequence of SEQ ID NO:17, 113, 146, 49, 80, and 178;
- CR1-gamma, selected from the amino acid sequence of SEQ ID NO:18, 114, 147, 50, 81, and 179;
- CR1-delta, selected from the amino acid sequence of SEQ ID NO:19, 115, 148, 51, 82, 180;
- RID-alpha, selected from the amino acid sequence of SEQ ID NO:20, 116, 149, 52, 83, and 181;
- RID-beta, selected from the amino acid sequence of SEQ ID NO:21, 117, 150, 53, 93, and 182;
- E3/14.7K, selected from the amino acid sequence of SEQ ID NO:28, 125, 158, 60, 84, and 189;
- and
- Fiber, selected from the amino acid sequence of SEQ ID NO:22, 118, 151, 54, 85 and 183.
33 more elements
Dependencies are inferred from the claim text.