Materials and methods for the delivery of biomolecules to cells of an organ
AssigneeVGX Pharmaceuticals, Inc.
InventorRuxandra Draghia-Akli
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Abstract
The present invention is directed towards materials and methods of delivering biomolecules to cells that define an organ, and the organ being in situ, and electroporating cells defining the organ by delivering to the cells an amount of energy effective to electroporate the cells. The present invention is also directed towards materials and methods of expressing heterologous polypeptides in organs of a subject and electroporating cells defining the organ by delivering to the cells an amount of energy effective to electroporate the cells.
Claims25
A method of delivering a biomolecule to a cell that defines an organ, the organ being in situ, comprising:
- delivering the biomolecule to the organ, and
- providing an amount of energy to the organ effective to electroporate the cells that define the organ.
A method of expressing a heterologous polypeptide in an organ of a subject, comprising:
- injecting a needle transdermally, transthoracically, or directly to the organ for delivery of an encoding nucleic acid molecule that encodes the heterologous polypeptide, and
- electroporating cells defining the organ by delivering to the cells an amount of energy effective to electroporate the cells.
A system adapted for the delivery of an encoding nucleic acid molecule to an organ of a mammal for the expression of a polypeptide from cells defining the organ, comprising:
- a needle for delivery of the encoding nucleic acid molecule to the organ, and
- an electorporation device capable of delivering pulses of energy at an intensity effective for electroporation of the cells of the organ.
25-28. (canceled)
Dependencies are inferred from the claim text.