Pico liter well holding device and method of making the same
AssigneeSeng Enterprises Ltd.
InventorsMordechai Deutsch, Assaf Deutsch
Life of this patent
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Abstract
Described herein are embossing methods for forming pico liter well arrays (and other structures), arrangements of such pico liter well arrays, for example, in arrays of pico liter well areas, and methods of forming templates for such embossing. Also described as wells with a refractive index similar to that of an aqueous medium used for supporting cells in the wells.
Claims30
1-11. (canceled)
A method of forming a template for a picowell array, comprising:
- (a) providing a form defining thereon a plurality of pico liter wells;
- (b) applying at least one apertured spacer above said form;
- (c) contacting said form with a settable material through said apertured spacer;
- and
- (d) removing said spacer and said set material from said form.
A method of forming a cell holding device with at least one pico liter well array, comprising:
- (a) providing a base covered with a hardening material, adapted to at least indirectly adhere to said base;
- (b) contacting said base with a die, said die defining at least one pico liter well region and said die adapted to not adhere to said hardening material;
- and
- (c) at least partially hardening said hardening material while in contact with said die.
-19. (canceled)
-36. (canceled)
A method of making a holding device for studying cells, comprising:
- providing a carrier plate;
- applying a first adhesive layer to the carrier plate;
- depositing a curable substrate on the adhesive layer;
- applying a second layer of adhesive to the substrate;
- attaching a wall structure to the second layer of adhesive;
- forming a multiplicity of pico liter wells in the substrate with a template;
- curing the substrate;
- and
- removing the template.
A method of making a holding device, comprising:
- providing a layer of curable substrate;
- disposing a wall structure on the layer of curable substrate;
- embossing a multiplicity of pico liter wells in a surface of the curable substrate;
- and curing the substrate.
42-43. (canceled)
A method of making a holding device for studying cells comprising:
- providing a carrier plate having a plurality of cavities surrounded by walls;
- depositing a curable substrate in each cavity;
- embossing a multiplicity of pico liter wells in the curable substrate with a die;
- and
- curing the substrate.
(canceled)
-51. (canceled)
A holding device for studying cells comprising a plurality of spaced apart pico liter well arrays, wherein said pico liter well arrays comprise embossed regions.
A holding device for studying cells comprising at least two defined regions:
- (a) a pico liter well array region including a plurality of pico liter wells;
- and
- (b) a non cell holding region in fluid communication with said pico liter well region,
- wherein said pico liter well array is embossed, and wherein fluid can be one or both of added and removed from said non cell holding region without disturbing cells in said pico liter wells.
Dependencies are inferred from the claim text.