Microfluidic cell culture device and method for using same
OwnerShuichi Takayama, Lourdes Marcella Cabrera +4inventors
InventorsShuichi Takayama, Lourdes Marcella Cabrera, Nobuyuki Futai, Yun Seok Heo, Gary Daniel Smith, Xiaoyue Zhu
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Abstract
Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.
Claims11
1-22. (canceled)
A microfluidic cell culture device comprising:
- a) a substrate;
- b) first and second reservoirs at least partially defined by the substrate, each of the reservoirs having upper and lower portions, the lower portions of the reservoirs containing a biological fluid and the upper portions of the reservoirs containing a cover fluid over the biological fluid;
- and
- c) one or more lower passages at least partially defined by the substrate, the lower passage fluidly communicating the biological fluid between the lower portions of the reservoirs.
32-37. (canceled)
Dependencies are inferred from the claim text.