Method of increasing tryptophan and nicotinamide levels in vivo
OwnerRonald W. Peroinventor
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Abstract
Novel methods of increasing the biosynthesis of aromatic amino acids and compositions containing the precursors of said aromatic amino acids or nicotinamide and methods of use thereof. In addition, methods of monitoring the therapeutic effects of an anti-oxidant therapy by measuring serum protein thiols as a surrogate or predictor of such therapy are disclosed.
Claims41
A precursor for an aromatic amino acid having the following formula:
- A-(X)n
- where A is selected from the group consisting of
- and anions, esters thereof;
- wherein R is H, C1-C3 alkyl, and
- X is selected from the group consisting of any monovalent, divalent, trivalent anions that can form salts or hydroxides or amines, and
- “n” is an integer or a fraction, wherein 1<n=10.
An animal model methodology for identifying an effective anti-oxidant therapeutic regimen for a disease state characterized by aromatic amino acid deficiency comprising the steps:
- (a) obtaining a mammal selected from the group consisting of mice, rats, rabbits, dogs, pigs, monkeys and humans,
- (b) determining a baseline value ratio for urinary tryptophan, nicotinamide, hippuric, kynurenine levels/tissue or blood thiol levels of said mammal,
- (c) administering to the mammal a composition comprising a precursor of an aromatic amino acid,
- (d) determining a value for the ratio of urinary tryptophan, nicotinamide, hippuric, kynurenine levels/tissue or blood thiol levels of said mammal after a cycle of the anti-oxidant treatment,
- (e) comparing the values obtained in steps (b) and (d), wherein a 30% increase over the baseline value indicates that the therapeutic regimen has been effective.
A method of testing the in vivo oxidative stress in a patient in need comprising
- (a) treating the patient with a composition comprising a precursor for an aromatic amino acid,
- (b) obtaining a sample of protein thiols from said patient,
- (c) determining the protein thiols levels in the sample,
- (d) measuring urinary levels of tryptophan or nicotinamide,
- (e) analyzing the thiol(s) levels in sample of step (c) in relation to the urinary levels of tryptophan or nicotinamide obtained in step (d) expressed as (d)/(c),
- wherein an increase in the value of said ratio is a predictor of low oxidative stress and down regulation of DNA repair.
A method of ascertaining a patient's susceptibility to a disease characterized by increased level of oxidative stress comprising
- (a) treating the patient with a composition comprising an aromatic amino acid precursor,
- (b) obtaining a body sample from said patient comprising protein thiol(s) levels,
- (c) determining the protein thiol(s) levels of the sample,
- (d) measuring urinary levels of tryptophan or nicotinamide levels,
- (e) analyzing the thiol(s) levels in sample of step (c) against the urinary levels of tryptophan or nicotinamide obtained in step (d) wherein a higher value against a predetermined normalized value obtained from a population suffering from said disease is the predictor of said individual to oxidative stress and down regulation of DNA repair.
A method for determining the risk for developing a disease characterized by deficiency in aromatic amino acids in an individual in need thereof, comprising the steps of:
- (a) obtaining levels of the individual's serum thiol(s) and levels of urinary tryptophan, nicotinamide or combinations or both,
- (b) establishing a value for the ratio of serum thiol(s)/urinary tryptophan, nicotinamide or combinations for said individual,
- (c) comparing the value of step (b) against a predetermined normalized levels of the same obtained from a population afflicted by said disease, wherein said comparison indicates the baseline value for the oxidative stress in said individual.
Dependencies are inferred from the claim text.