Recombinant poly-glutamic acid depolymerases
OwnerArthur M. Friedlander, Angelo Scorpio +1inventors
InventorsArthur M. Friedlander, Angelo Scorpio, Donald J. Chabot
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Abstract
In this application is described isolated, recombinant CapD and recombinant PghP for use in digesting capsule comprising polyglutamate polymers and for treatment of infections caused by bacilli having a polyglutamate capsule, such as anthrax.
Claims36
An isolated recombinant capsule depolymerase, CapD.
A method for producing recombinant CapD protein comprising:
- growing a host cell according to claim transformed with a vector expressing CapD in a suitable culture medium,
- causing expression of said vector sequence as defined above under suitable conditions for production of soluble protein and,
- lysing said transformed host cells and recovering said CapD.
A composition for degrading a poly-γ-D-glutamic polymer, said composition comprising recombinant CapD.
An isolated recombinant poly-γ-glutamate hydrolase, PghP.
A method for producing recombinant PghP protein comprising:
- growing a host cell according to claim transformed with a vector expressing PghP in a suitable culture medium,
- causing expression of said vector sequence as defined above under suitable conditions for production of soluble protein and,
- lysing said transformed host cells and recovering said PghP.
A composition for degrading a poly-γ-DL-glutamic polymer, said composition comprising recombinant PghP.
Dependencies are inferred from the claim text.