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US 2010/0216205 A1Published application
Reagents and Methods for Cyanobacterial Production of Bioplastics and Biomaterials
AssigneeArizona Board of Regents, a body corporate acting for and on behalf of Arizona State University
InventorsHatem Mohammed, Wim Vermaas
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Abstract
The present invention provides reagents and methods for biomaterial production from cyanobacteria.
Claims42
A method for producing biomaterials, comprising:
- (a) culturing cyanobacterial host cells that are deficient in Slr1125 expression;
- (b) harvesting the cyanobacterial host cells;
- and
- (c) preparing biomaterials from the harvested cyanobacterial host cells.
1 more element
An isolated recombinant nucleic acid, comprising:
- (a) a first nucleic acid comprising an inducible cyanobacterial promoter;
- and
- (b) a second nucleic acid operably linked to the first nucleic acid, wherein the second nucleic acid encodes an inhibitory nucleic acid complementary to a target nucleic acid sequence that encodes an amino acid sequence of a polypeptides selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8.
A recombinant cyanobacterial host cell, comprising:
- (a) a deficiency in Slr1125 expression;
- and
- (b) one or more of the following recombinantly generated phenotypes:
- (i) a deficiency in cyanophycin production;
- (ii) a deficiency in poly-β-hydroxyalkanoate (PHA) production;
- (iii) overexpression of NAD synthetase;
- (iv) overexpression of NAD+ kinase;
- (v) deficiency in cyanophycin synthetase expression;
- (vi) deficiency in Slr1993 expression;
- (vii) deficiency in cyanophycinase expression;
- (viii) deficiency in PHB production;
- (ix) deficiency in Slr 1994 expression;
- (x) deficiency in Slr 1829 expression;
- and
- (xi) deficiency in Slr 1830 expression.
12 more elements
Dependencies are inferred from the claim text.