Attenuated recombinant newcastle disease virus and vaccine containing the same

A strain of Newcastle disease virus,

• comprising nucleotide sequences encoding NP, P, M, and L proteins of a low-pathogenic Newcastle disease virus that has an amino acid sequence represented by following Formula 1 at 113th to 116th positions of F protein;
• and nucleotide sequences encoding F, and HN proteins of a high-pathogenic Newcastle disease virus that has an amino acid sequence represented by following Formula 2 at 113th to 116th positions of the F protein,
• wherein the F protein coding sequence comprised in the strain is characterized by substitution of the codon encoding the 115th amino acid of the F protein of the high-pathogenic Newcastle disease virus with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU;
• aspartic acid codons consisting of GAC and GAU;
• phenylalanine codons consisting of UUC and UUU;
• isoleucine codons consisting of AUC and AUU;
• leucine codons consisting of UUA and UUG;
• serine codons consisting of UCA, UCC, UCG and UCU;
• threonine codons consisting of ACC and ACU;
• valine codons consisting of GUA, GUC, GUG and GUU;
• and tyrosine codons consisting of UAC and UAU:
• 113-X1X2X3X4-116 Formula 1
• wherein
• X1, X3 and X4 are independently arginine (R) or lysine (K), and
• X2 is selected from the group consisting of alanine, isoleucine, leucine, methionine, phenylalanine, proline, tryptophan, valine, asparagine, cysteine, glutamine, glycine, serine, theronine, tyrosine, aspartic acid, glutamic acid, arginine, histidine, and lysine.
• 113-X4X5X7X8-116 Formula 2
• wherein
• X5, X6 and X7 are independently selected from the group consisting of alanine, isoleucine, leucine, methionine, phenylalanine, proline, tryptophan, valine, asparagine, cysteine, glutamine, glycine, serine, theronine, tyrosine, aspartic acid, glutamic acid, arginine, histidine and lysine, and X5 and X7 are not both arginine (R) or lysine (K) at the same time, and
• X8 is arginine (R) or lysine (K).