# US 2010/0169998 A1 · Use of Silk Number Assay to Screen for Genes Enhancing Corn Yield

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- Number: US 2010/0169998 A1
- Document ID: US20100169998A1
- Status: Published application
- Kind: A1
- Application number: 12/647,572
- Filed: 2009-12-28
- Published: 2010-07-01
- Earliest claimed: 2008-12-29
- Assignees: Pioneer Hi-Bred International, Inc.
- Inventors: Guofu Li; Junli Ji
- Web page: https://patentgrep.com/patent/US20100169998A1

## CPC

- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The invention provides a rapid and efficient method and assay for monitoring yield enhancement in a plant using a measure of silk number. The plant is transformed with a prospective gene associated with yield enhancement. The transformed plant is grown along with non-transformed control plants until silk growth is apparent. The change in the number of silks in the transformed plant correlated to the change in yield for the plant. Therefore, changes in yield enhancement can be estimated in the transformed plant prior to harvest of mature plant tissues.

## Claims

### Claim 1

A method for measuring enhanced yield in maize, comprising:
a. transforming a plant or its ancestor with a construct comprising a yield enhancement gene operably linked to a polynucleotide encoding a polypeptide which increases silk number,
b. growing the plants under plant growing conditions until silks develop,
c. counting the number of silks present in the ear, and
d. correlating the number of transformed plant silks with control plant silks to determine which plants have statistically significantly increased silk number.

### Claim 2 (depends on 1)

The method of claim 1, wherein the plant is an inbred, a hybrid or any plant transformed with a polynucleotide.

### Claim 3 (depends on 1)

The method of claim 1, wherein a plurality of plants are screened.