# US 2010/0167997 A1 · Method for stimulation collagen synthesis and/or kgf expression > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2010/0167997 A1 - Document ID: US20100167997A1 - Status: Published application - Kind: A1 - Application number: 11/815,307 - Filed: 2006-01-24 - Published: 2010-07-01 - Earliest claimed: 2005-02-01 - Assignees: aTyr Pharma, Inc. - Inventors: Sung-Hoon Kim - Web page: https://patentgrep.com/patent/US20100167997A1 ## CPC - A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof - A61P 17/00: Drugs for dermatological disorders - A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like - A61P 17/16: Emollients or protectives, e.g. against radiation - A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 - A61Q 19/08: Anti-ageing preparations - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS ## Abstract The present invention relates to a method for stimulating collagen synthesis and/or KGF expression, and more particularly, to a method for stimulating collagen synthesis and/or KGF expression using the AIMP1 or its fragment. The AIMP1 or its fragment can be effectively used for the stimulation of collagen synthesis and/or KGF expression in a subject in need thereof, the treatment of skin aging in the subject, the treatment of the flaccid and/or wrinkled skin in the subject, the promoting the smoothing and/or firming of the skin in the subject, the treatment of adverse cutaneous effects of menopause in the subject, and the treatment of adverse effects of menopause on the collagen. ## Claims ### Claim 1 A method for stimulating collagen synthesis and/or KGF (keratinocyte growth factor) expression in vitro or in vivo, comprising administering to a subject in need thereof an effective amount of one selected from the group consisting of: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% to the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 2 A method for treating skin aging, comprising administering to a subject in need thereof an effective amount of one selected from the group consisting of: (a) an isolated polypeptide having an amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having an amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 3 A method for treating the flaccid and/or wrinkled skin, comprising administering to a subject in need thereof an effective amount of one selected from the group consisting of: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 4 A method for promoting the smoothing and/or firming of the skin, comprising administration to a subject in need thereof of an effective amount of one selected from the group consisting of: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 5 A method for treating adverse cutaneous effects of menopause, comprising administration to a subject in need thereof of an effective amount of one selected from the group consisting of: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 6 A method for treating adverse effects of menopause on the collagen, comprising administration to a subject in need thereof of an effective amount of one selected from the group consisting of: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 7 (depends on 1, 2, 3, 4, 5, 6) The method of any one of claims 1 to 6, wherein the polypeptide of (b) is selected from the group consisting of SEQ ID NO: 24, SEQ ID NO: 25 and SEQ ID NO: 26. ### Claim 8 (depends on 1, 2, 3, 4, 5, 6) The method of any one of claims 1 to 6, wherein the fragment of (c) is a peptide comprising the amino acid sequence set forth in SEQ ID NO: 12 and consisting of at least 192 amino acids. ### Claim 9 (depends on 8) The method of claim 8, wherein the fragment of (c) is a peptide consisting of the amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID No: 13, SEQ ID NO: 14 and SEQ ID NOs: 27 to 34. ### Claim 10 A composition for stimulating collagen synthesis and/or KGF expression, the composition comprising as an active ingredient one selected from the group consisting of: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 11 A cosmetic composition for preventing skin aging, the composition comprising as an active ingredient one selected from the group consisting of: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 12 (depends on 10, 11) The composition of claim 10 or claim 11, wherein the polypeptide of (b) is selected from the group consisting of SEQ ID NO: 24, SEQ ID NO: 25 and SEQ ID NO: 26. ### Claim 13 (depends on 10, 11) The composition of claim 10 or claim 11, wherein the fragment of (c) is a peptide comprising the amino acid sequence set forth in SEQ ID NO: 12 and consisting of at least 192 amino acids. ### Claim 14 (depends on 13) The composition of claim 13, wherein the fragment of (c) is a peptide consisting of the amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID No: 13, SEQ ID NO: 14 and SEQ ID NOs: 27 to 34. ### Claim 15 Use of any one selected from the group consisting of the following, for preparing a composition for stimulating collagen synthesis and/or KGF expression, the composition comprising the one as an active ingredient: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO:1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 16 Use of any one selected from the group consisting of the following proteins, for preparing a cosmetic composition for preventing skin aging, the composition comprising the one as an active ingredient: (a) an isolated polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (b) an isolated polypeptide having the amino acid sequence homology of at least 70% with the polypeptide (a); and (c) a fragment of the polypeptide (a) or (b). ### Claim 17 (depends on 15, 16) The use of claim 15 or claim 16, wherein the polypeptide of (b) is selected from the group consisting of SEQ ID NO: 24, SEQ ID NO: 25 and SEQ ID NO: 26. ### Claim 18 (depends on 15, 16) The use of claim 15 or claim 16, wherein the fragment of (c) is a peptide comprising the amino acid sequence set forth in SEQ ID NO: 12 and consisting of at least 192 amino acids. ### Claim 19 (depends on 18) The use of claim 18, wherein the fragment of (c) is a peptide consisting of the amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID No: 13, SEQ ID NO: 14 and SEQ ID NOs: 27 to 34.