# US 2010/0167955 A1 · Microarray including layer comprising dna molecule and method of manufacturing the same

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- Number: US 2010/0167955 A1
- Document ID: US20100167955A1
- Status: Published application
- Kind: A1
- Application number: 12/510,625
- Filed: 2009-07-28
- Published: 2010-07-01
- Earliest claimed: 2008-12-26
- Assignees: Samsung Electronics Co., Ltd.
- Inventors: Sun-ok Jung
- Web page: https://patentgrep.com/patent/US20100167955A1

## CPC

- C40B 50/14: Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
- C40B 30/04: by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)

## Abstract

A microarray with excellent sensitivity for detecting target materials during biological assays, and a method of manufacturing the same.

## Claims

### Claim 1

A microarray comprising:
a substrate;
a spot region formed on the substrate, wherein the spot region comprises a probe material capable of binding to a target material; and
a layer formed on a region of the substrate other than where the spot region is formed, wherein the layer comprises DNA molecules, each comprising at least two nucleotides.

### Claim 2

A microarray comprising:
a substrate;
a layer formed on the substrate wherein the layer comprises DNA molecules, each comprising at least two nucleotides; and
a spot region formed on the layer, wherein the spot region comprises a probe material capable of binding to a target material.

### Claim 3 (depends on 1)

The microarray of claim 1, wherein the binding of the probe material to the target material is performed at a temperature greater than about 19° C.

### Claim 4 (depends on 1)

The microarray of claim 1, wherein the probe material comprises one selected from a group consisting of DNA, RNA, nucleotide, nucleoside, protein, polypeptide, peptide, amino acid, carbohydrate, enzyme, antibody, antigen, receptor, virus, substrate, ligand, membrane, and the like and a combination comprising at least one of the foregoing probe materials.

### Claim 5 (depends on 1)

The microarray of claim 1, wherein the nucleotide comprises a base selected from a group consisting of adenine, guanine, thymine, and cytosine.

### Claim 6 (depends on 1)

The microarray of claim 1, wherein the DNA molecules comprise 2 to 6 nucleotides.

### Claim 7 (depends on 1)

The microarray of claim 1, wherein the substrate is in the form of a plane, a bead, or a sphere.

### Claim 8 (depends on 1)

The microarray of claim 1, wherein the substrate is selected from a group consisting of silicon, glass, metal, plastic, and ceramic.

### Claim 9

A method of manufacturing a microarray, the method comprising:
preparing a substrate;
forming a spot regions on the substrate, wherein the spot region comprises a probe material capable of binding to a target material; and
forming a layer on a region of the substrate other than where the spot region is formed, wherein the layer comprises DNA molecules, each comprising at least two nucleotides.

### Claim 10

A method of manufacturing a microarray, the method comprising:
preparing a substrate;
forming a layer on the substrate, wherein the layer comprises DNA molecules, each comprising at least two nucleotides; and
forming a spot region on the layer, wherein the spot region comprises a probe material capable of binding to a target material on the layer.

### Claim 11 (depends on 9)

The method of claim 9, wherein the substrate is in the form of a plane, a bead, or a sphere.

### Claim 12 (depends on 9)

The method of claim 9, wherein the substrate is selected from a group consisting of silicon, glass, metal, plastic, and ceramic.

### Claim 13 (depends on 9)

The method of claim 9, wherein the nucleotide comprises a base selected from a group consisting of adenine, guanine, thymine, and cytosine.

### Claim 14 (depends on 9)

The method of claim 9, wherein each of the DNA molecules comprises 2 to 6 nucleotides.

### Claim 15 (depends on 9)

The method of claim 9, wherein the probe material comprises one selected from a group consisting of DNA, RNA, nucleotide, nucleoside, protein, polypeptide, peptide, amino acid, carbohydrate, enzyme, antibody, antigen, receptor, virus, substrate, ligand, membrane, and the like and a combination comprising at least one of the foregoing probe materials.

### Claim 16 (depends on 2)

The microarray of claim 2, wherein the binding of the probe material to the target material is performed at a temperature greater than about 19° C.

### Claim 17 (depends on 2)

The microarray of claim 2, wherein the probe material comprises one selected from a group consisting of DNA, RNA, nucleotide, nucleoside, protein, polypeptide, peptide, amino acid, carbohydrate, enzyme, antibody, antigen, receptor, virus, substrate, ligand, membrane, and the like and a combination comprising at least one of the foregoing probe materials.

### Claim 18 (depends on 2)

The microarray of claim 2, wherein the nucleotide comprises a base selected from a group consisting of adenine, guanine, thymine, and cytosine.

### Claim 19 (depends on 2)

The microarray of claim 2, wherein the DNA molecules comprise 2 to 6 nucleotides.

### Claim 20 (depends on 2)

The microarray of claim 2, wherein the substrate is in the form of a plane, a bead, or a sphere.

### Claim 21 (depends on 2)

The microarray of claim 2, wherein the substrate is selected from a group consisting of silicon, glass, metal, plastic, and ceramic.

### Claim 22 (depends on 10)

The method of claim 10, wherein the substrate is in the form of a plane, a bead, or a sphere.

### Claim 23 (depends on 10)

The method of claim 10, wherein the substrate is selected from a group consisting of silicon, glass, metal, plastic, and ceramic.

### Claim 24 (depends on 10)

The method of claim 10, wherein the nucleotide comprises a base selected from a group consisting of adenine, guanine, thymine, and cytosine.

### Claim 25 (depends on 10)

The method of claim 10, wherein each of the DNA molecules comprises 2 to 6 nucleotides.

### Claim 26 (depends on 10)

The method of claim 10, wherein the probe material comprises one selected from a group consisting of DNA, RNA, nucleotide, nucleoside, protein, polypeptide, peptide, amino acid, carbohydrate, enzyme, antibody, antigen, receptor, virus, substrate, ligand, membrane, and the like and a combination comprising at least one of the foregoing probe materials.