# US 2010/0167950 A1 · Microarray chip and method of fabricating for the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2010/0167950 A1 - Document ID: US20100167950A1 - Status: Published application - Kind: A1 - Application number: 12/649,993 - Filed: 2009-12-30 - Published: 2010-07-01 - Earliest claimed: 2008-12-31 - Assignees: National Health Research Institute - Inventors: Jyh-Lyh Juang; Yi-You Huang; Po-Cheng Chen - Web page: https://patentgrep.com/patent/US20100167950A1 ## CPC - G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics} - B01L 3/5085: {for multiple samples, e.g. microtitration plates} - B01J 2219/00317: Microwell devices, i.e. having large numbers of wells - B01J 2219/00509: Microcolumns - B01J 2219/0072: Organic compounds - B01J 2219/00743: Cells - B01L 2300/0636: Integrated biosensor, microarrays - B01L 2300/0819: Microarrays; Biochips - B01L 2300/0829: Multi-well plates; Microtitration plates - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS ## Abstract The present invention provides a microarray chip for use in the analysis of various sample types. The microarray chips disclosed herein generally comprise a substrate covered with a coating material comprising a photoresist material, wherein the coating material is patterned to comprise a plurality of microstructures such as microwells and/or microcolumns. Methods for preparing and utilizing the microarray chips of the invention are further provided. The microarray chips of the instant invention find particular use in high-throughput assays. ## Claims ### Claim 1 A microarray chip comprising: (a) a substrate; and (b) a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microstructures on the substrate. ### Claim 2 (depends on 1) The microarray chip of claim 1, wherein the substrate is quartz. ### Claim 3 (depends on 1) The microarray chip of claim 1, wherein the substrate is glass. ### Claim 4 (depends on 1) The microarray chip of claim 1, wherein the at least one commercially available photoresist material comprises NANO™ SU-8 2-15, NANO™ SU-8 50-100, NANO™ SU-8 2000 series photoresist, NANO™ SU-8 3000 series photoresist and KMPR® 1000 series photoresist films. ### Claim 5 (depends on 1) The microarray chip of claim 1, wherein the microstructures are microwells. ### Claim 6 (depends on 1) The microarray chip of claim 1, wherein the microstructures are microcolumns. ### Claim 7 (depends on 6) The microarray chip of claim 6, wherein the microcolumns further comprises micromachined microcolumns. ### Claim 8 (depends on 6) The microarray chip of claim 6, wherein the microcolumns further comprises microelectromechanical systems (MEMS) fabricated microcolumns. ### Claim 9 (depends on 1) The microarray chip of claim 1, wherein the microstructures include a combination of microwells on one surface of the substrate and microcolumns on another surface of the substrate. ### Claim 10 A platform for analyzing binding of a probe to a sample comprising: (a) an array chip comprising at least one substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells; (b) a means for applying the probe to the sample in the microwells; and (c) a means for detecting any binding of the probe and the sample and any phenotypic change resulting from binding of the probe and the sample. ### Claim 11 (depends on 10) The platform of claim 10, wherein the at least one commercially available photoresist material comprises NANO™ SU-8 2-15, NANO™ SU-8 50-100, NANO™ SU-8 2000 series photoresist, NANO™ SU-8 3000 series photoresist and KMPR® 1000 series photoresist films. ### Claim 12 (depends on 10) The platform of claim 10, wherein the substrate is quartz. ### Claim 13 (depends on 10) The platform of claim 10, wherein the substrate is glass. ### Claim 14 (depends on 10) The platform of claim 10, wherein the means for applying the probe comprises a plurality of microcolumns. ### Claim 15 A drug screening method comprising: (a) providing a microarray chip, wherein the microarray chip comprises a substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells; (b) culturing a target cell in the microwells; (c) dispensing a candidate drug into the microwells; and (d) detecting any binding of the candidate drug to the target cell and any phenotypic change resulting from binding of the candidate drug and the target cells. ### Claim 16 (depends on 15) The method of claim 15, wherein the at least one commercially available photoresist material comprises NANO™ SU-8 2-15, NANO™ SU-8 50-100, NANO™ SU-8 2000 series photoresist, NANO™ SU-8 3000 series photoresist and KMPR® 1000 series photoresist films. ### Claim 17 (depends on 15) The method of claim 15, wherein the candidate drug is dispensed from a plurality of microcolumns on the substrate. ### Claim 18 A method for analyzing binding of a probe to a sample comprising: (a) providing a microarray chip, wherein the microarray chip comprises a substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells; (b) applying the probe to the sample in the microwells; and (c) detecting any binding of the probe to the sample and any phenotypic change resulting from binding of the probe to the sample. ### Claim 19 (depends on 18) The method of claim 18, wherein the at least one commercially available photoresist material comprises NANO™ SU-8 2-15, NANO™ SU-8 50-100, NANO™ SU-8 2000 series photoresist, NANO™ SU-8 3000 series photoresist and KMPR® 1000 series photoresist films. ### Claim 20 (depends on 18) The method of claim 18, wherein the probe is applied from a plurality of microcolumns on one surface of the substrate.