# US 2010/0167402 A1 · Human serum for cell culture > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2010/0167402 A1 - Document ID: US20100167402A1 - Status: Published application - Kind: A1 - Application number: 12/705,298 - Filed: 2010-02-12 - Published: 2010-07-01 - Earliest claimed: 2004-11-19 - Assignees: JMS Co., Ltd. - Inventors: Koji SUZUKI; Seishin Tanaka - Web page: https://patentgrep.com/patent/US20100167402A1 ## CPC - C12N 5/0018: {Culture media for cell or tissue culture (media for specific animal cell type C12N5/06)} - A61K 35/16: Blood plasma; Blood serum (umbilical cord blood A61K35/51) - C12N 2501/135: Platelet-derived growth factor [PDGF] - C12N 2501/15: Transforming growth factor beta (TGF-β) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract It is intended to provide a serum which contains a large amount of growth factors capable of efficiently promoting the growth of stem cells. A human serum for cell culture which shows a residual ratio of platelets remaining within 20 minutes after blood collection in relation to the whole amount of the platelets is 0% to 20%, and a release ratio of cell growth factors is 20% to 100%. ## Claims ### Claim at position 1 1-8. (canceled) ### Claim 9 A method of producing human serum for cell culture, the method comprising: an activation acceleration process of contacting blood and a blood coagulation accelerating substance having a surface area of 0.1 to 25 mm2 per ml of said blood, and shaking; and a centrifugation process of separating serum by centrifugation from the blood that has undergone the activation acceleration process. ### Claim 10 (depends on 9) A method of producing human serum for cell culture according to claim 9, wherein the blood coagulation accelerating substance is glass beads. ### Claim 11 (depends on 10) A method of producing human serum for cell culture according to claim 10, wherein a surface of the glass beads is formed of a layer comprising a silicon dioxide compound using at least one selected from of glass, silica, diatomaceous earth, and kaolin. ### Claim 12 (depends on 10) A method of producing human serum for cell culture according to claim 10, wherein the shape of the glass beads is substantially spherical. ### Claim 13 (depends on 9) A method of producing human serum for cell culture according to claim 9, comprising a blood storing process of storing the blood in a blood reservoir containing the blood coagulation accelerating substance, before the activation acceleration process. ### Claim 14 (depends on 10) A method of producing human serum for cell culture according to claim 10, comprising a blood storing process of storing the blood in a blood reservoir containing the blood coagulation accelerating substance, before the activation acceleration process. ### Claim 15 (depends on 13) A method of producing human serum for cell culture according to claim 13, wherein, in the centrifugation process, centrifugation is performed on the blood reservoir in which blood that has undergone the activation acceleration process is stored. ### Claim 16 (depends on 14) A method of producing human serum for cell culture according to claim 14, wherein, in the centrifugation process, centrifugation is performed on the blood reservoir in which blood that has undergone the activation acceleration process is stored. ### Claim 17 (depends on 9) A method of producing human serum for cell culture according to claim 9, comprising a derivation process of containing the serum in a component storage part without contact with outside air, after the centrifugation process. ### Claim 18 (depends on 10) A method of producing human serum for cell culture according to claim 10, comprising a derivation process of containing the serum in a component storage part without contact with outside air, after the centrifugation process. ### Claim 19 (depends on 13) A method of producing human serum for cell culture according to claim 13, comprising a derivation process of containing the serum in a component storage part without contact with outside air, after the centrifugation process. ### Claim 20 (depends on 14) A method of producing human serum for cell culture according to claim 14, comprising a derivation process of containing the serum in a component storage part without contact with outside air, after the centrifugation process. ### Claim 21 (depends on 15) A method of producing human serum for cell culture according to claim 15, comprising a derivation process of containing the serum in a component storage part without contact with outside air, after the centrifugation process. ### Claim 22 (depends on 16) A method of producing human serum for cell culture according to claim 16, comprising a derivation process of containing the serum in a component storage part without contact with outside air, after the centrifugation process. ### Claim 23 (depends on 17) A method of producing human serum for cell culture according to claim 17, wherein, in the derivation process, serum separated in the centrifugation process is contained in a component storage part via a tube connected in an airtight manner with the blood reservoir. ### Claim 24 (depends on 18) A method of producing human serum for cell culture according to claim 18, wherein, in the derivation process, serum separated in the centrifugation process is contained in a component storage part via a tube connected in an airtight manner with the blood reservoir. ### Claim 25 (depends on 19) A method of producing human serum for cell culture according to claim 19, wherein, in the derivation process, serum separated in the centrifugation process is contained in a component storage part via a tube connected in an airtight manner with the blood reservoir. ### Claim 26 (depends on 20) A method of producing human serum for cell culture according to claim 20, wherein, in the derivation process, serum separated in the centrifugation process is contained in a component storage part via a tube connected in an airtight manner with the blood reservoir. ### Claim 27 (depends on 21) A method of producing human serum for cell culture according to claim 21, wherein, in the derivation process, serum separated in the centrifugation process is contained in a component storage part via a tube connected in an airtight manner with the blood reservoir. ### Claim 28 (depends on 22) A method of producing human serum for cell culture according to claim 22, wherein, in the derivation process, serum separated in the centrifugation process is contained in a component storage part via a tube connected in an airtight manner with the blood reservoir.