# US 2010/0167398 A1 · Method of culturing pluripotent stem cells using extracellular matrix from fetal membrane-derived cells

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2010/0167398 A1
- Document ID: US20100167398A1
- Status: Published application
- Kind: A1
- Application number: 12/646,156
- Filed: 2009-12-23
- Published: 2010-07-01
- Earliest claimed: 2008-12-24
- Assignees: Riken
- Inventors: Yoshiki SASAI; Tomoko NAGASE; Morio UENO; Yonehiro KANEMURA
- Web page: https://patentgrep.com/patent/US20100167398A1

## CPC

- C12N 5/0606: {Pluripotent embryonic cells, e.g. embryonic stem cells [ES] (embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696)}
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C12N 5/0696: {Artificially induced pluripotent stem cells, e.g. iPS}
- C12N 2533/92: Amnion; Decellularised dermis or mucosa
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Provided are a method of culturing pluripotent stem cells in the presence of a decidua-derived cell or an extracellular matrix derived from the cell, that enables safe and efficient maintenance culture and derivation of pluripotent stem cells; a culture agent for pluripotent stem cells, that comprises a decidua-derived cell or an extracellular matrix derived from the cell; and other means for developing or performing the method.

## Claims

### Claim 1

A method of culturing pluripotent stem cells in the presence of decidua-derived cells or an extracellular matrix derived from the cell.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the decidua-derived cell is a mesenchymal cell.

### Claim 3 (depends on 1)

The method according to claim 1, wherein both the decidua-derived cell and the pluripotent stem cells are derived from a mammal of the same species.

### Claim 4 (depends on 3)

The method according to claim 3, wherein the mammal is a human.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the pluripotent stem cells are human ES cells or human iPS cells.

### Claim 6

A culture agent for pluripotent stem cells, containing decidua-derived cells or an extracellular matrix derived from the cell.

### Claim 7 (depends on 6)

The agent according to claim 6, wherein the decidua-derived cell is a mesenchymal cell.

### Claim 8 (depends on 6)

The agent according to claim 6, wherein both the decidua-derived cell and the pluripotent stem cells are derived from a mammal of the same species.

### Claim 9 (depends on 8)

The agent according to claim 8, wherein the mammal is a human.

### Claim 10 (depends on 6)

The agent according to claim 6, wherein the pluripotent stem cells are human ES cells or human iPS cells.

### Claim 11

A container for culturing pluripotent stem cells, coated with an extracellular matrix from decidua-derived cells.

### Claim 12 (depends on 11)

The container according to claim 11, wherein the decidua-derived cell is a mesenchymal cell.

### Claim 13 (depends on 11)

The container according to claim 11, wherein both the decidua-derived cell and the pluripotent stem cells are derived from a mammal of the same species.

### Claim 14 (depends on 13)

The container according to claim 13, wherein the mammal is a human.

### Claim 15 (depends on 11)

The container according to claim 11, wherein the pluripotent stem cells are human ES cells or human iPS cells.

### Claim 16

A kit for culturing pluripotent stem cells, comprising (i) and (ii) below:
(i) a culture agent for pluripotent stem cells, comprising decidua-derived cells or an extracellular matrix derived from the cell; and
(ii) an explanatory document stating that the kit should be used, or can be used, for culturing pluripotent stem cells.

### Claim 17

A kit for culturing pluripotent stem cells, comprising (i) and (ii) below:
(i) a container for culturing pluripotent stem cells, coated with an extracellular matrix from decidua-derived cells; and
(ii) an explanatory document stating that the kit should be used, or can be used, for culturing pluripotent stem cells.